ISSN:
1432-1017
Keywords:
Amphipathic α-helix
;
Channel-forming peptide
;
Hydrophilic surface map
;
Membrane protein
;
Simulated annealing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Ion channels may be formed by bundles of amphipathic α-helices aligned parallel to one another and spanning a lipid bilayer membrane, with the hydrophilic faces of the helices lining a central pore. In order to provide insight into the packing of such helices in bundles, a method has been developed to evalute hydrophilic surface maps of amphipathic α-helices and to display these surfaces in a readily interpretable form. The procedure is based upon empirical energy calculations of interactions of a water molecule with an amphipathic α-helix. The method has been applied to three channel-forming peptides: Staphylococcal δ-toxin; alamethicin; and a synthetic leucine- and serine-containing peptide. Particular emphasis is placed upon the effects of sidechain conformational flexibility on hydrophilic surface maps. Å family of models of the δ-toxin; helix is generated by a simulated annealing procedure. The results of hydrophilic surface map analyses provide more exact definition of the centre of the hydrophilic face of amphipathic helices, and of the variation of the position of the centre in response to changes in sidechain conformation. This information is used to define families of preliminary models for a given ion channel, as is illustrated for δ-toxin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00180261
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