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  • 1
    ISSN: 1432-2048
    Keywords: Key words: Chloroplast ; DNA-binding protein (puri fication) ; Operon ( psaA) ; Spinacia (chloroplast) ; Trans-acting factor ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We have previously shown the presence in chloroplasts of sequence-specific DNA-binding proteins that interact specifically with two regions located downstream and upstream from the 5′-transcription start site of the plastid psaA-psaB-rps14 operon. As part of an effort to elucidate the regulatory mechanism of plastid transcription during plant development, we report here the purification and characterization of the chloroplast DNA-binding protein from spinach (Spinacia oleracea L. var. spinosa Ashers et Graeden) leaves that specifically recognizes sequences between positions +64 to +83 relative to the transcription start site. This DNA-binding protein has been highly purified from chloroplasts by using a combination of high-salt extraction, ammonium sulfate precipitation, heparin-agarose chromatography, and sequence-specific DNA-affinity chromatography. The protein exhibited an apparent molecular weight of 59–60 kDa on the basis of gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Southwestern blot analysis further indicated that this DNA-binding protein is dimeric and composed of two ≈31-kDa subunits. We discuss the properties of this protein in relation to the known chloroplast DNA-binding factors for plastid gene expression.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 41 (1995), S. 2661-2663 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 42 (1996), S. 1706-1714 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Experimental studies of polymer melt flow in the filling and post-filling stages of the injection molding process were performed using the sequential injection of transparent and colored polystyrene resin. Effects of fountain flow in the filling stage, geometrical factors caused by edges and corners, as well as flow through contractions and expansions, were identified. Significant polymer melt flow which increases with increased packing pressure was observed in the post-filling process. The melt flow is more concentrated around the gate area than away from the gate. It was also found that the polymer melt flows across the gap center, resulting in partial annihilation of the weld line. Simulations based on the control-volume/finite-element method employed within each gapwise layer combined with the dual-filling-parameter technique were developed to trace the advancements in melt fronts for both skin and core materials. Numerical simulations show reasonable consistency with experimental results in both skin and core material distribution. If the edge effect is taken into account using a shape factor as a geometrical correction, the simulation accuracy is further improved.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
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