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  • Chemistry  (1)
  • Flavodoxin  (1)
  • HCANH  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Doubly sensitivity-enhanced 3D TOCSY-HSQC (1996)
    Springer
    Journal of biomolecular NMR 8 (1996), S. 319-330 
    Details
    ISSN: 1573-5001
    Keywords: B0 gradients ; INEPT ; COS-INEPT ; Multidimensional NMR ; Sensitivity enhancement ; TOCSY-HSQC ; HSQC ; Flavodoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Recently, strategies for double sensitivity enhancement in heteronuclear three-dimensional NMR experiments were introduced (Krishnamurthy, V.V. (1995) J. Magn. Reson., B106, 170–177; Sattler et al. (1995) J. Biomol. NMR, 6, 11–22; Sattler et al. (1995) J. Magn. Reson., B108, 235–242). Since a sensitivity enhancement of a factor 21/2 can be achieved for each indirect dimension, nD spectra can theoretically be enhanced up to a factor of 2((n-1)/2). We propose and analyze a doubly enhanced three-dimensional TOCSY-HSQC sequence. The application of the doubly enhanced three-dimensional {15N, 1H} TOCSY-HSQC sequence is shown for uniformly 13C-/15N- and 15N-labeled samples of the relatively large Azotobacter vinelandii flavodoxin II (179 amino acids). The main factors that contribute to the final signal-to-noise enhancement have been systematically investigated. The sensitivity enhancement obtained for the doubly enhanced TOCSY-HSQC pulse sequence as compared to the standard (unenhanced) version is close to the theoretically expected factor of two.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410330
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  • 2
    Electronic Resource
    Electronic Resource
    A high-resolution HCANH experiment with enhanced sensitivity via multiple quantum line narrowing (1999)
    Springer
    Journal of biomolecular NMR 14 (1999), S. 169-174 
    Details
    ISSN: 1573-5001
    Keywords: calmodulin ; HCANH ; multiple quantum line narrowing ; NMR assignment ; resolution ; SEF2-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We report a 3D constant-time HCANH experiment (CTSL-HCANH) that uses the slower relaxation of multiple-quantum coherence to increase sensitivity and provides high Cα resolution. In this experiment the Hα of the (Hα, Cα) multiple quanta are selectively spin locked, so that Hα chemical shift evolution and 1 H-1H J-dephasing become ineffective during the relatively long delay needed for Cα to N coherence transfer. As compared to an HCANH experiment that uses Cα single-quantum coherence, an average enhancement of 20% was observed on calmodulin in complex with the binding domain of the transcription factor SEF2-1. Compared to CBCANH the signal intensity is approximately twice as good. The favorable relaxation properties of multiple quanta, together with the outstanding Cα resolution, make the experiment a very good complement to CBCANH and CBCA(CO)NH for sequential assignment of larger proteins for which deuteration is not yet necessary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008308307363
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Rotational diffusion of short DNA fragments in polyacrylamide gels: An electric birefringence study (1986)
    New York : Wiley-Blackwell
    Biopolymers 25 (1986), S. 2173-2186 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Using electric birefringence we have examined the rotational diffusion of five short DNA fragments (55 to 256 base pairs) both in polyacrylamide gels as a function of gel concentration and in solution. The length dependence of the measured rotational relaxation times in the gels is in good agreement with the prediction from the Odijk theory for the dynamics of slightly flexible rods in a network. The rotational relaxation times were found to depend on the gel concentration, contrary to the prediction from the Odijk theory. Possible reasons for this observation are discussed. The birefringence decay curves for DNA fragments in the gel were single exponential only at small electric field strength.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360251110
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    Paper (German National Licenses)
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