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  • Chemistry  (2)
  • Functional characterization  (1)
  • Key words Airway obstruction  (1)
  • 1
    Digitale Medien
    Digitale Medien
    CO2 laser subtotal arytenoidectomy and posterior true and false cordotomy in the treatment of post-thyroidectomy bilateral laryngeal fixation in adduction (1999)
    Springer
    European archives of oto-rhino-laryngology and head & neck 256 (1999), S. 291-295 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Key words Airway obstruction ; Vocal cord palsy ; CO2 laser surgery ; Transoral arytenoidectomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract A total of 39 patients with bilateral post-thyroidectomy vocal cord paralysis in adduction underwent CO2 laser subtotal arytenoidectomies with removal of the posterior third of the false and true vocal cords. Total airway resistance (R tot) evaluated before and 4–10 months after surgery showed marked preoperative impairment before and significant improvement after surgery (P 〈 0.05). In five patients revision surgery was performed due to a progressive impairment of respiratory function. A variable degree of voice breathiness was observed after surgery; the maximum phonation time mean values were lower than normal and peak sound pressure levels 63 ± 5 dB. In three cases aspiration was present in the first postoperative days, but swallowing dysfunctions disappeared within 1 week. Subtotal arytenoidectomy with removal of the posterior third of the true and false vocal folds was found to be a satisfactory surgical treatment for bilateral vocal cord paralysis in adduction. However, further research is still needed to define the surgical procedure able to balance respiratory, phonatory and sphincteric functions optimally.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004050050248
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  • 2
    Digitale Medien
    Digitale Medien
    Morphological and functional characteristics of human temporal-bone cell cultures (1983)
    Springer
    Cell & tissue research 229 (1983), S. 505-513 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Bone-cell cultures ; Hormonal stimulation ; Calcium uptake ; Functional characterization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Morphology and calcium metabolism have been studied on five different cell cultures from human normal adult temporal-bone biopsies obtained during five stapedectomies. Control cell cultures were obtained from normal human skin. Four different cell types were observed in the bone biopsies: 1) osteoblast-like cells; 2) osteoclast-like cells; 3) fibroblast-like cells; 4) intermediate cells. However, morphology by itself is inadequate for clear differentiation of the four cell types. Hormonal stimulation with calcitonin and dibutyryl-cAMP in presence of 45Ca++ showed a clear-cut difference in 45Ca++ uptake between cultured cells deriving from bone and skin. Functional responses to hormonal stimulation are therefore more specific than cell shape and morphology in differentiating fibroblasts from bone cells. Since responses to hormonal stimulation confirm that temporal-bone cell cultures actually contain bone cells, such cultures seem to be a good experimental model for the study of bone morphology and physiology.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00207694
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  • 3
    Digitale Medien
    Digitale Medien
    PTH induces modification of transductive events in otosclerotic bone cell cultures (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 11 (1993), S. 257-261 
    Details
    ISSN: 0263-6484
    Schlagwort(e): PTH ; adenylate cyclase ; Ca2+ ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: We studied the effect of PTH (10-100 nM) on transductive mechanisms (adenylate cyclase activity, Ca2+ metabolism, IP3 levels) in cell cultures derived from normal and otosclerotic human bone fragments. The cultured cells were osteoblast-like but with calcitonin-receptors still present and with PTH receptors coupled with the adenylate cyclase system.The results showed that PTH activated adenylate cyclase and increased the intracellular Ca2+ levels with qualitative and quantitative differences between the two cellular populations. In particular, otosclerotic cells responded less to hormone stimulation, which is in accord with the current hypothesis of a desensitization of the receptor/enzyme complex associated with the pathological status.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cbf.290110406
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  • 4
    Digitale Medien
    Digitale Medien
    Human bone cell cultures: A new model for studying the mechanism of action of calcitonin (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 3 (1985), S. 179-184 
    Details
    ISSN: 0263-6484
    Schlagwort(e): Human bone cell cultures ; calcitonin action ; 45Ca incorporation ; cyclic nucleotides ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: An investigation on cell cultures obtained from temporal human bone fragments showed that they provide a suitable model for studying the mechanism involved in calcitonin action on bone cells. Furthermore they demonstrated: (1) a transitory increase in 45Ca uptake that returned to control values ten minutes after the hormone was added; (2) a relation between 45Ca uptake and increased cAMP concentrations when these were measured at the same time intervals; (3) a reproduction of the salmon calcitonin (sCT) effect after incubation of the cultures with either db-cAMP or db-cGMP and (4) inhibition of 45Ca uptake and parallel decrease in cAMP levels with propanol.These results suggest that in human bone cell cultures, sCT acts as a temporary promoter of 45Ca uptake, probably by activating an adenylate-cyclase system through a β-receptor.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cbf.290030304
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