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  • 1
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 26 (1901), S. 452-453 
    ISSN: 0863-1778
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 149-150 
    ISSN: 0935-6304
    Keywords: Liquid Chromatography, HPLC ; Ion pair reverse phase gradient technique starting at low pH ; Viral associated glycosoaminoglycans, G2 and G4 separated ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 177-178 
    ISSN: 0935-6304
    Keywords: Liquid Chromatography, HPLC ; Analysis of methods for proteins and peptide separation and yield ; Reverse phase, ion exchange, molecular exclusion ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: New methods for peptide separation are being developed. Resolution of peptide mixtures on HPLC with good reproducibility indicates the progress that has already been made in development of supports with useful separation capabilities. At present adsorptive characteristics of the stationary phases may place limitations on the applicability of the technique to underivatized peptides. Hopefully, the application of ionpairing techniques and the development of new support materials will expand the range of application for protein and peptide purification.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 970-977 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Actual laboratory data obtained from steady-state Dunaliella tertiolecta cultures grown under a wide range of photon flux densities were used in a simple model to calculate daily production in a conventional algal mass culture system. In spite of large physiological and biochemical variations between low-light- (LL) and high light- (HL) adapted cultures, the overall calculated daily productivity is almost identical for both strains grown at optimal conditions. When production of fine biochemicals is considered, however, a hypothetical HL strain, which cannot shade adapt, is advantageous. Based on biochemical and biophysical analysis of D. tertiolecta responses to growth irradiance levels, specific targets are defined for genetic manipulation to enhance productivity in algal mass culture systems. The targets identified are (1) amplification of the carboxylation enzyme ribulose-1,5-bisphosphate carboxylase-oxygenase relative to the electron transport complexes, which should increase photosynthetic capacity at light saturation, and (2) enlargement of the light-harvesting complexes by varying their pigment composition in order to increase light harvesting at low photon flux densities.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 4 (1958), S. 418-422 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 25 (1900), S. 265-269 
    ISSN: 0863-1778
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 67 (1966), S. 207-215 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An attempt is made to summarize and synthesize salient points from the conference. Considering the immunoglobulin-synthesizing and hemoglobin-synthesizing cell systems in parallel, first attention is given to aspects of cellular differentiation that deal with specialization in the formation of the predominant molecular products, the immunoglobulins and the hemoglobins. Primary structural considerations point to similar genetic mechanisms as the basis for phylogenetic diversity and similarities within each of the two classes of macromolecules, and to similar restrictions on the sorts of amino acid substitutions that can be tolerated while retaining the funcional integrity of the molecules. In the immunoglobulins, the appearance of “variable regions” in the component polypeptide chains, presumably associated with the diverse specificities required of these molecules in order that they may serve their functions, provides additional challenges for interpretation, and the significance of present knowledge in this context is evaluated. Other aspects of cellular differentiation are approached by way of the developmental shifts in molecular products found in both systems, and the explanations that have been suggested for these shifts. A striking difference in the two systems, the phenomenon of allele exclusion in immunoglobulin control, is discussed. The review concludes with discussions of more complicated aspects of differentiation in these cell series: the role of hormones and other humoral substances, particularly with regard to the immunoglobulin-synthesizing system, deriving from the thymus; parallels among cell-lineage patterns in the various hematopoietic series; and evidences of the causes of senescence in the immunoglobulin-synthesizing system.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 26 (1984), S. 65-73 
    ISSN: 0730-2312
    Keywords: lyphokines ; interleukin 1 ; macrophage cell line ; protein complex ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The murine macrophage-derived cell line P388D1 secretes the lymphokine inter-leukin 1 (IL-1) when stimulated by a variety of agents. When stimulated by bacterial lipopolysaccharide (LPS) the cells release IL-1 in both high and low molecular weight (m.w.) forms. The proportion of high m.w. IL-1 is reduced when IL-1-containing supernatants are concentrated by ammonium sulfate precipitation subsequent to hollow-fiber filtration. The high m.w. form can be converted to the low m.w. form by proteolysis, reduction and alkylation, or chromatography in a dissociating solvent. The low m.w. form remains as such, even when reconcentrated in fetal calf serum-containing medium. The high m.w. form thus likely consists of a complex between low m.w. IL-1 and another protein secreted by the P388D1 cell line.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Laser microprobe mass spectrometry (LMMS) has been applied systematically to a variety of organic polyfunctional molecules, covering a wide range of structures and polarities. The microprobe generally offers a combination of desorption under relatively mild conditions with abundant fragmentation. We attempted an empirical approach by tentative hypotheses about desorption and ionization in LMMS to consistently rationalize the detected fragments. The complementary nature of structural data, carried by positive and negative ions, is characteristic for LMMS results of non-ionic compounds. The analysis of salts represents, traditionally, an ultimate test case for soft methods in organic mass spectrometry. Hence, by a selected series of compounds, we have tried to assess to which extent the presence of preformed ions becomes an asset for LMMS analysis and affects the amount, the accessibility or the distribution of organic information between positive and negative fragments.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Laser microprobe mass spectrometry (LMMS) has been used to systematically study polyfunctional molecules, covering a wide range of structure and polarity. The knowledge about the mechanisms actually involved for desorption and ionization (DI) of organics by laser microbeam irradiation of solid samples at high-power density is rather limited. Therefore we have elaborated a set of tentative hypotheses about DI in LMMS, permitting consistent rationalization of detected signals. The technique apparently combines desorption under mild conditions, shown by the release of intact thermolabiles, with extensive fragmentation. Structural data are typically distributed between cations and anions. Interpretation of negative-ion detection mode mass spectra often represents intricate problems, partly due to the lack of sustaining background information from conventional mass spectrometry. Selected examples are presented to illustrate the occurrence of electron capture ionization, the role of heteroatoms in the formation of negative ions and the tendency to undergo complex skeletal rearrangements. Although LMMS was originally aimed at microprobe applications, it has been found to be a valuable tool in organic mass spectrometry.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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