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  • Chemistry  (10)
  • Monoclonal antibody  (3)
  • Rice  (3)
  • learning flexible concepts
  • 1
    ISSN: 0009-8981
    Keywords: Interstitial collagenase ; Monoclonal antibody ; Sandwich enzyme immunoassay ; Tissue inhibitors of metalloproteinases
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0009-8981
    Keywords: Monoclonal antibody ; Sandwich enzyme immunoassay ; Tissue inhibitor of metalloproteinases-2
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Machine learning 8 (1992), S. 5-43 
    ISSN: 0885-6125
    Keywords: Concept learning ; learning imprecise concepts ; inductive learning ; learning flexible concepts ; two-tiered concept representation ; flexible matching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract This paper describes a method for learning flexible concepts, by which are meant concepts that lack precise definition and are context-dependent. To describe such concepts, the method employs a two-tiered representation, in which the first tier captures explicitly basic concept properties, and the second tier characterizes allowable concept's modifications and context dependency. In the proposed method, the first tier, called Base Concept Representation (BCR), is created in two phases. In phase 1, the AQ-15 rule learning program is applied to induce a complete and consistent concept description from supplied examples. In phase 2, this description is optimized according to a domain-dependent quality criterion. The second tier, called the inferential concept interpretation (ICI), consists of a procedure for flexible matching, and a set of inference rules. The proposed method has been implemented in the POSEIDON system, and experimentally tested on two real-world problems: learning the concept of an acceptable union contract, and learning voting patterns of Republicans and Democrats in the U.S. Congress. For comparison, a few other learning methods were also applied to the same problems. These methods included simple variants of exemplar-based learning, and an ID-3-type decision tree learning, implemented in the ASSISTANT program. In the experiments, POSEIDON generated concept descriptions that were both, more accurate and also substantially simpler than those produced by the other methods.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Machine learning 8 (1992), S. 5-43 
    ISSN: 0885-6125
    Keywords: Concept learning ; learning imprecise concepts ; inductive learning ; learning flexible concepts ; two-tiered concept representation ; flexible matching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract This paper describes a method for learningflexible concepts, by which are meant concepts that lack precise definition and are context-dependent. To describe such concepts, the method employs atwo-tiered representation, in which the first tier captures explicitly basic concept properties, and the second tier characterizes allowable concept's modifications and context dependency. In the proposed method, the first tier, calledBase Concept Representation (BCR), is created in two phases. In phase 1, the AQ-15 rule learning program is applied to induce a complete and consistent concept description from supplied examples. In phase 2, this description is optimized according to a domain-dependent quality criterion. The second tier, called theinferential concept interpretation (ICI), consists of a procedure forflexible matching, and a set of inference rules. The proposed method has been implemented in the POSEIDON system, and experimentally tested on two real-world problems: learning the concept of an acceptable union contract, and learning voting patterns of Republicans and Democrats in the U.S. Congress. For comparison, a few other learning methods were also applied to the same problems. These methods included simple variants of exemplar-based learning, and an ID-3-type decision tree learning, implemented in the ASSISTANT program. In the experiments, POSEIDON generated concept descriptions that were both, more accurate and also substantially simpler than those produced by the other methods.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 19 (1999), S. 128-132 
    ISSN: 1432-203X
    Keywords: Key words Zygote ; Egg cell ; Plant regeneration ; Individual culture ; Rice ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple mechanical method has been developed which allows the routine isolation of unfertilized and fertilized egg cells from ovules of Japonica and Indica rice varieties. In the experiments described, the majority of the egg cells and zygotes survived the isolation procedure when the donor plants were in a vigorous state. About 40% of the surviving zygotes underwent sustained development when cultured in Millicell inserts with a non-morphogenic rice feeder-cell culture. Nearly all zygote-derived callus cultures regenerated multiple shoots, which could be subsequently rooted with high efficiency. Zygote-derived plantlets matured to fertile plants when transplanted to soil. So far, about 80 independent plants each from the Japonica variety 'Taipei309' and the Indica variety 'IR58' have been regenerated. The potential of this single-cell regeneration system for marker gene-free transformation is discussed.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2242
    Keywords: Key words DNA markers ; Drought resistance ; QTLs ; Rice ; Root penetration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Evaluation of root traits in rainfed lowland rice is very difficult. Molecular genetic markers could be used as an alternative strategy to phenotypic selection for the improvement of rice root traits. This research was undertaken to map QTLs associated with five root traits using RFLP and AFLP markers. Recombinant inbred lines (RILs) were developed from two indica parents, IR58821–23-B-1–2-1 and IR52561-UBN-1–1-2, that were adapted to rainfed lowland production systems. Using wax-petrolatum layers to simulate a hardpan in the soil, 166 RILs were evaluated for total root number (TRN), penetrated root number (PRN), root penetration index (RPI, the ratio of PRN to TRN), penetrated root thickness (PRT) and penetrated root length (PRL) under greenhouse conditions during the summer and the fall of 1997. A genetic linkage map of 2022 cM length was constructed comprising 303 AFLP and 96 RFLP markers with an average marker space of 5.0 cM. QTL analysis via interval mapping detected 28 QTLs for these five root traits, which were located on chromosomes 1, 2, 3, 4, 6, 7, 10 and 11. Individual QTLs accounted for between 6 and 27% of the phenotypic variation. Most of the favorable alleles were derived from the parent IR58821–23-B-1–2-1, which was phenotypically superior in root traits related to drought resistance. Three out of six QTLs for RPI were detected in both summer and fall experiments and they also were associated with PRN in both experiments. Out of eight QTLs for RPT, five were common in both seasons. Two genomic regions on chromosome 2 were associated with three root traits (PRN, PRT and RPI), whereas three genomic regions on chromosomes 2 and 3 were associated with two root traits (PRT and RPI). Two QTLs affecting RPI and two QTLs affecting PRT were also found in similar genomic regions in other rice populations. The consistent QTLs across genetic backgrounds and the common QTLs detected in both experiments should be good candidates for marker-assisted selection toward the incorporation of root traits in a drought resistance breeding program, especially for rainfed lowland rice.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2242
    Keywords: Key words Plasma membrane H+-ATPase gene ; Salt stress ; Salt-tolerant mutant ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Plasma membrane (PM) H+-ATPase plays an important role in the establishment and maintenance of ion homeostasis. To investigate its expression in the rice salt-tolerant mutant M-20 and the original variety 77–170 during salt stress, a cDNA fragment corresponding to the PM H+-ATPase gene was obtained by PCR from rice japonica variety 77–170 and designated as OSA3. Sequence analysis of OSA3 revealed its high homology with two other published PM H+-ATPase genes, OSA1 and OSA2, in rice. Southern-blot analysis detected a RFLP between M-20 and 77–170, and one copy of the OSA3 gene was mapped to a position on rice chromosome 12 where a salt tolerance QTL was closely located. The expression of the PM H+-ATPase gene, as revealed by the OSA3 fragment, was compared between M-20 and 77–170. The results demonstrated that M-20 shoots accumulated less transcripts than 77–170 shoots at a later stage of salt treatment, and M-20 showed high expression at 300 mM NaCl while 77–170 reached its maximum at 200 mM NaCl. In roots, the difference in the level of the PM H+-ATPase gene expression between stressed and non-stressed plants was substantially greater in M-20 than that in 77–170. The relative abundance of PM H+-ATPase gene transcripts in M-20 roots may indicate the active role of this gene in the strict control of Na+ and Cl+ uptake into root symplast and apoplast, and further translocation into the shoot, hence leading to the reduced gene expression of M-20 shoots under salt-stress conditions.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Optically active isotactic polychloral was prepared in which the optical activity comes exclusively from molecular asymmetry (i.e., helical conformation). Molecular asymmetry requires a high conformational energy barrier for the polymer backbone, and an asymmetric initiator to induce a predominance of one helical screw-sense. Polychloral meets the criteria. Asymmetric initiators used to obtain optically active polychloral include tetramethyl ammonium (+)- or (-)-0-acetylmandelate, tetramethylammonium (+)- or (-)-0-methylmandelate, the lithiumalkoxides of methyl (+)- or (-)-mandelate, lithium cholesten-3β-oxide, lithium cholestan-3β-oxide, and lithium (+)- or (-)-2-octanoxide. Using the above initiators at 0.5 mol %, a maximum specific rotation of [α] D25 = 5000 was obtained for polychloral. Errors in specific rotation were typically ±7%.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 351-359 
    ISSN: 0006-3592
    Keywords: bioreactor ; high density ; insect cells ; perfusion ; Sf9 ; ultrasonic filter ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The baculovirus/insect cell expression system has provided a vital tool to produce a high level of active proteins for many applications. We have developed a very high-density insect cell perfusion process with an ultrasonic filter as a cell retention device. The separation efficiency of the filter was studied under various operating conditions. A cell density of over 30 million cells/mL was achieved in a controlled perfusion bioreactor and cell viability remained greater than 90%. Sf9 cells from a high-density culture and a spinner culture were infected with two recombinant baculoviruses expressing genes for the production of human chitinase and monocyte-colony inhibition factor. The protein yield on a cell basis from infecting high-density Sf9 cells was the same as or higher than that from the spinner Sf9 culture. Virus production from the high-density culture was similar to that from the spinner culture. The results show that the ultrasonic filter did not affect insect cells' ability to support protein expression and virus production following infection with baculovirus. The potential applications of the high-density perfusion culture for large-scale protein expression from Sf9 cells are also highlighted. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:351-359, 1998.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Long-chain unsaturated fatty acids (UFA) can easily be converted on a microgram scale to the corresponding 2-alkenyl-4,4-dimethyloxazolines by condensation with 2-amino-2-methylpropanol (AMP). These modified molecules with a ‘hidden’ carboxyl group have been proved to be a class of useful derivatives for gas chromatography/mass spectrometry (GC/MS) of UFA mixtures. While possessing very good GC characteristics, the title compounds show regular, well-recognizable diagnostic ion peaks of the double bond position in the chain. Detailed description of the method as well as electron impact (E1) mass spectra of derivatives resulting from mono-, di and polyenoic (maximally containing six double bonds) acids are presented.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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