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  • 1
    Electronic Resource
    Electronic Resource
    Fertile plant regeneration from protoplasts of meadow fescue (Festuca pratensis Huds.) (1993)
    Springer
    Plant cell reports 12 (1993), S. 95-100 
    Details
    ISSN: 1432-203X
    Keywords: forage grasses ; Festuca pratensis ; suspension cultures ; protoplasts ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Suspension cultures from mature embryo-derived compact callus were initiated in seven meadow fescue (Festuca pratensis Huds.) cultivars. Four to six months after initiation, embryogenic suspension cultures with a moderate growth rate were established from three of them (cvs. Barmondo, Belimo and Leopard). These suspension cultures showed the capacity, maintained over six months, to regenerate green plants which could be grown to maturity under greenhouse conditions. Morphogenic suspension cultures from single genotypes of three F. pratensis cultivars (cvs. Barmondo, Belimo and Leopard) yielded large numbers of protoplasts, which upon culture in agarose beads using nurse cells formed microcalli with an overall plating efficiency in the range of 10-3 to 10-4. Mature plants were reproducibly regenerated and established in soil, from such protoplasts during a period of six months. The regeneration of fertile plants from protoplasts derived from suspension cultures of meadow fescue and its implications on gene transfer technology for this species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00241942
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of genetic stability of plants regenerated from suspension cultures and protoplasts of meadow fescue (Festuca pratensis Huds.) (1993)
    Springer
    Plant cell reports 12 (1993), S. 101-106 
    Details
    ISSN: 1432-203X
    Keywords: Festuca pratensis ; suspension cultures ; protoplasts ; plant regeneration ; somaclonal variation ; genetic fidelity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cytological and molecular analysis was performed to assess the genetic uniformity and true-to-type character of plants regenerated from 20 week-old embryogenic suspension cultures of meadow fescue (Festuca pratensis Huds.), and compared to protoplastderived plants obtained from the same cell suspension. Cytological variation was not observed in a representative sample of plants regenerated directly from the embryogenic suspensions and from protoplasts isolated therefrom. Similarly, no restriction fragment length polymorphisms (RFLPs) were detected in the mitochondrial, plastid and nuclear genomes in the plants analyzed. Randomly amplified polymorphic DNA markers (RAPDs) have been used to characterise molecularly a set of mature meadow fescue plants regenerated from these in vitro cultures. RAPD markers using 18 different short oligonucleotide primers of arbitrary nucleotide sequence in combination with polymerase chain reaction (PCR) allowed the detection of pre-existing polymorphisms in the donor genotypes, but failed to reveal newly generated variation in the protoplast-derived plants compared to their equivalent suspensionculture regenerated materials. The genetic stability of meadow fescue plants regenerated from suspension cultures and protoplasts isolated therefrom and its implications on gene transfer technology for this species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00241943
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells (1997)
    Springer
    Plant cell reports 16 (1997), S. 379-384 
    Details
    ISSN: 1432-203X
    Keywords: Forage and turf grasses ; Italian ryegrassLolium multiflorum ; Microprojectile bombardment ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic forage-type Italian ryegrass (Lolium multiflorum Lam.) plants have been obtained by microprojectile bombardment of embryogenic suspension cells using a chimeric hygromycin phosphotransferase (hph) gene construct driven by riceActl 5′ regulatory sequences. Parameters for the bombardment of embryogenic suspension cultures with the particle inflow gun were partially optimized using transient expression assays of a chimericβ-glucuronidase (gusA) gene driven by the maizeUbi1 promoter. Stably transformed clones were recovered with a selection scheme using hygromycin in liquid medium followed by a plate selection. Plants were regenerated from 33% of the hygromycin-resistant calli. The transgenic nature of the regenerated plants was demonstrated by Southern hybridization analysis. Expression of the transgene in transformed adult Italian ryegrass plants was confirmed by northern analysis and a hygromycin phosphotransferase enzyme assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146777
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  • 4
    Electronic Resource
    Electronic Resource
    Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells (1997)
    Springer
    Plant cell reports 16 (1997), S. 379-384 
    Details
    ISSN: 1432-203X
    Keywords: Key words Forage and turf grasses ; Italian ryegrass ; Lolium multiflorum ; Microprojectile bombardment ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic forage-type Italian ryegrass (Lolium multiflorum Lam.) plants have been obtained by microprojectile bombardment of embryogenic suspension cells using a chimeric hygromycin phosphotransferase (hph) gene construct driven by rice Act1 5′ regulatory sequences. Parameters for the bombardment of embryogenic suspension cultures with the particle inflow gun were partially optimized using transient expression assays of a chimeric β-glucuronidase (gusA) gene driven by the maize Ubi1 promoter. Stably transformed clones were recovered with a selection scheme using hygromycin in liquid medium followed by a plate selection. Plants were regenerated from 33% of the hygromycin-resistant calli. The transgenic nature of the regenerated plants was demonstrated by Southern hybridization analysis. Expression of the transgene in transformed adult Italian ryegrass plants was confirmed by northern analysis and a hygromycin phosphotransferase enzyme assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146777
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Synthesis of linear polyformal and copoly(carbonate formal) from polycarbonate (1992)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 30 (1992), S. 299-304 
    Details
    ISSN: 0887-624X
    Keywords: polyformal ; polycarbonate ; copoly(carbonate formal) ; phase-transfer catalyst ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A linear, high molecular weight polyformal is readily synthesized by direct transformation of commercially available polycarbonate. The reaction is best carried out in dibromomethane with 8.0 equiv. of potassium hydroxide pellets in the presence of a phase-transfer catalyst at 90-95°C. A random copoly(carbonate formal) can also be obtained simply by reducing the amount of potassium hydroxide used in the reaction. The molecular weight of the resulting polymer is governed by the starting polycarbonate.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1992.080300214
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Synthesis of poly(arylene ether)s based on 9,9-bis(3,5-diphenyl-4-hydroxyphenyl)fluorene (1991)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 29 (1991), S. 1045-1052 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The synthesis of a novel bisphenol, 9,9-bis(3,5-diphenyl-4-hydroxyphenyl)fluorene, in high yield by the transalkylation of 9,9-bis(4-hydroxyphenyl)fluorene is described. Three poly(arylene ether)s based on this hindered phenol were prepared with molecular weights (Mn) ranging from 34 800 to 51 300 and inherent viscosities ranging from 0.27 to 0.43 dL/g. The polymers have Tg's of 236-262°C and did not lose weight below 350°C, with 10% weight loss recorded above 550°C. They are readily soluble in chlorinated solvents such as methylene chloride, chloroform, and 1,2-dichloroethane at room temperature. Attempts to synthesize an analogous monomer, bis (3,5-diphenyl-4-hydroxyphenyl)diphenylmethane, are described.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1991.080290712
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  • 7
    Electronic Resource
    Electronic Resource
    The retention behaviour of conjugated bile acids in reversed phase high performance liquid chromatography (1992)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 6 (1992), S. 255-257 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The retention behaviour of conjugated bile acids has been studied in a reversed phase high performance liquid chromatographic (RP-HPLC) system by using the mixture of methanol and aqueous phosphate buffer as the mobile phase. The retentions of the conjugates in RP-HPLC have been found to be mainly controlled by the glycine and taurine groups. The selectivity between five different glycine and taurine conjugated bile acids is a constant in RP-HPLC. This selectivity has been used for peak identification in the practical separation of conjugated bile acids.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130060512
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  • 8
    Electronic Resource
    Electronic Resource
    High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases (1993)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 7 (1993), S. 139-142 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer-assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130070307
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    Paper (German National Licenses)
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