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  • Chemistry  (2)
  • differential DNA polymerase activity  (1)
  • 1
    Electronic Resource
    Electronic Resource
    RNase-sensitive DNA polymerase activity in cell fractions and mutants of Neurospora crassa (1980)
    Springer
    Biochemical genetics 18 (1980), S. 743-753 
    Details
    ISSN: 1573-4927
    Keywords: RNase-senitive DNA polymerase activity ; RNA ; DNA ; Neurospora crassa ; differential DNA polymerase activity ; cell fractions and mutants of N. crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract RNase-sensitive DNA polymerase activity (RSDP) was tested in different cell fractions of Neurospora crassa cell types and its morphological mutants. This RSDP was found localized in the microsomal pellet fraction and absent in the purified nuclear pellets isolated from different N. crassa cell types: conidia, germinated conidia, and mycelia. This enzyme is capable of synthesizing a DNA product only in the presence of all four deoxyribonucleoside-5′-triphosphates and Mg2+. Removal of RNA from the pellet fraction by RNase strongly inhibited the DNA synthesis. The endogenous synthesis of DNA in the microsomal pellet fraction was associated with the formation of an RNA:DNA hybrid as analyzed by Cs2SO4 equilibrium density gradient centrifugation. The DNA product after alkali hydrolysis hybridizes with the RNA isolated from the same pellet fraction, as analyzed by elution from hydroxylapatite column at 60 C. This DNA product did not hybridize with poly(A). A few mutants tested showed this RNase-sensitive DNA polymerase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484590
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on process variables for natural fiber composites - effect of polyesteramide polyol as interfacial agent (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 35 (1988), S. 973-985 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A systematic study of the process variables for polyester composites based on different natural fibers like jute, sisal, ramie, and pineapple has been made with a view to determine the most suitable processing conditions for such composites. Effect of polyesteramide polyol (PEAP) as interfacial agent in such composites has also been discussed. Fracture modes of jute/polyester (JP) composite sample having different fiber volume fractions have been examined by scanning electron microscope (SEM). Fracture surfaces of untreated and PEAP-treated JP composite samples have been examined in SEM, both before and after 72 h immersion in boiling water, to study the nature of interfacial bonding on fiber treatment with PEAP. A qualitative improvement in bonding in case of PEAP-treated JP composites is evident from SEM photomicrographs. This is also supported by physicomechanical properties of the composite samples.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1988.070350412
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  • 3
    Electronic Resource
    Electronic Resource
    A dynamic mechanical study on unidirectional carbon fiber-reinforced polypropylene composites (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 51 (1994), S. 831-840 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Fiber-reinforced polymer composites show high specific strength and stiffness. The alignment of reinforcing fibers results in anisotropy of the material. This anisotropic behavior has been studied through dynamic mechanical analysis of unidirectional carbon fiber-reinforced polypropylene (CFRPP) composites measured in both parallel and transverse directions to fiber arrangement. Several parameters such as storage modulus (E′), loss modulus (E″), loss factor or damping factor (tan δ), and complex viscosity (MU*) have been determined over a wide range of frequencies and at a fixed temperature. Relaxation and retardation spectra have been constructed for these composites. Modulus enhancement occurs due to stiffness imparted by the fiber and efficient stress transfer at the interface. Relaxation of the polymer matrix ceases with increase in the volume fraction of the fibers. α′-relaxation is observed for the composite having a 13% volume fraction of fibers and is ascribed to relaxation in the crystalline phase where the additional crystallinity arises out of the transcrystalline growth at the fiber-matrix interface. There exists a good correlation between theroretical curves with the experimental ones. Relaxation and retardation spectra and the dynamic parameters determined for these composites show a good correlation with the volume fraction of fibers as well as the direction of the applied load. © 1994 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1994.070510506
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