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  • 1
    Electronic Resource
    Electronic Resource
    Purification and properties of α-glucose 1-phosphate-forming trehalose phosphorylase from a basidiomycete,Pleurotus ostreatus (2000)
    Springer
    Mycoscience 41 (2000), S. 607-613 
    Details
    ISSN: 1618-2545
    Keywords: basidiomycete ; α-glucose 1-phosphate formation ; Pleurotus ostreatus ; trehalose ; trehalose phosphorylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pleurotus ostreatus produced a high activity of α-glucose 1-phosphate (α-Glc 1-P) forming trehalose phosphorylase in vegetative mycelia and fruit-bodies. The enzyme was purified to homogeneity from the fruit-bodies by a procedure involving ammonium sulfate fractionation, DEAE-cellulose column chromatographies and cellulose phosphate column chromatographies. The enzyme catalyzes both the phosphorolysis of trehalose to produce α-Glc 1-P and glucose, and the synthesis of trehalose. It was not active toward other α- or β-glucosyl disaccharides and polysaccharides. The optimum pH was 7.0 for phosphorolysis and 6.4 for synthesis of trehalose. The Km values for trehalose and Pi in phospholytic reaction were 75 mM and 4.2 mM, respectively. Those for glucose and α-Glc 1-P in synthetic reaction were 505 mM and 38 mM, respectively. The estimated molecular mass by the sedimentation equilibrium method using an ultracentrifuge was 120 kDa. The molecular mass of the subunit (61 kDa) by SDS-polyacrylamide gel electrophoresis suggested that the enzyme was a dimer of two identical subunits. The addition of glycerol higher than 25% into the enzyme solution stabilized its activity. The removal of phosphorus ions from the enzyme solution, by means of dialysis or electrophoresis, caused inactivation of the enzyme, probably by dissociation of the holoenzyme into the subunit proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02460927
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  • 2
    Electronic Resource
    Electronic Resource
    Novel method of preparing Ag-Hg alloy on polyacrylamide films and their structure (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 29 (1984), S. 795-802 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A novel method is proposed of preparing thin Ag-Hg alloy on PAAm film surface at room temperature: The film of interest is formed by holding PAAm aqueous solution with AgNO3 in Hg-saturated atmosphere. Two kinds of films, one of which is a conductor and the other an insulator, can be selectively formed with pH-controlled PAAm solution by ammonia. The conducting surface is assigned to the α phase of Ag-Hg alloy by means of X-ray analysis. Potentiometric titration and IR spectral studies suggest the existence of PAAm-Ag+ complexes. On the basis of their structure and the oxidation and reduction potential of Ag+ and Hg2+, the mechanism of film formation is also discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1984.070290307
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  • 3
    Electronic Resource
    Electronic Resource
    λ-Type BETS salts containing a mixed halide gallium anion, GaXx Y4-x⊖ [X, Y = F, Cl, Br; BETS = Bis(ethylenedithio)tetraselenafulvalene] (1996)
    Weinheim : Wiley-Blackwell
    Advanced Materials 8 (1996), S. 812-815 
    Details
    ISSN: 0935-9648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/adma.19960081009
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