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  • 1
    Electronic Resource
    Electronic Resource
    High-level expression of secreted glycoproteins in transformed lepidopteran insect cells using a novel expression vector (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 60 (1998), S. 656-663 
    Details
    ISSN: 0006-3592
    Keywords: protein expression ; secreted glycoproteins ; insect cells ; cell transformation ; silkmoth actin gene promoter ; lepidoptera ; baculovirus enhancer ; baculovirus transcriptional activator ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An expression cassette for continuous high-level expression of secreted glycoproteins by transformed lepidopteran insect cells has been developed as an alternative to baculovirus and mammalian cell expression systems. The expression cassette utilizes the promoter of the silkmoth cytoplasmic actin gene to drive expression from foreign gene sequences, and also contains the ie-1 transactivator gene and the HR3 enhancer region of BmNPV to stimulate gene expression. Using an antibiotic-resistance selection scheme, we have cloned a Bm5 (silkmoth) cell line overexpressing the secreted glycoprotein juvenile hormone esterase (JHE-KK) at levels of 190 mg/L in batch suspension cultures. A baculovirus (AcNPV) expressing the same gene under the control of the p10 promoter of AcNPV produced only 4 mg/L active JHE in static cultures of infected Sf21 cells. A cloned Bm5 cell line overexpressing a soluble isoform of the α-subunit of the granulocyte-macrophage colony stimulating factor receptor (solGMRα) was also generated and produced five times more solGMRα in static cultures than a cloned BHK cell line obtained by transformation with a recombinant expression cassette utilizing the human cytomegalovirus (CMV) enhancer-promoter system. Finally, we show that recombinant protein expression levels in transformed Bm5 cells remain high in serum-free media, that expression is stable even in the absence of antibiotic selection, and that lepidopteran cells other than Bm5 may be used equally efficiently with this new expression cassette for producing recombinant proteins. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 656-663, 1998.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    New crown ether resins prepared by cationic template polymerization (1994)
    Weinheim : Wiley-Blackwell
    Macromolecular Chemistry and Physics 195 (1994), S. 391-399 
    Details
    ISSN: 1022-1352
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The synthesis of several dibenzo-18-crown-6 derivatives, and their acid catalyzed incorporation into crosslinked styrene and 4-methoxystyrene based copolymers is described. Some of these reactions were carried out in the presence of chiral ammonium cations acting as non-covalent templates. The polymers have been characterized by IR, and by 1H and 13C solution state and magic angle spinning NMR.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1994.021950201
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Monitoring of lamination processes in an autoclave with fiber-optic infrared spectroscopy (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 56 (1995), S. 667-675 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Curing reactions of certain laminates involve a series of chemical structure changes which occur with increasing temperature and/or pressure. This paper describes the use of a midinfrared chalcogenide fiber to monitor the lamination of polymer prepregs in an autoclave. The fiber optic was used both as a wave guide and as a sensing element for acquiring evanescent wave spectra. The spectra were measured by embedding the fiber optic in a polymer prepreg package. The polymer prepregs studied include epoxy, polyimide, and bismaleimide triazine (BT) resins. The degree of cure and an understanding of the kinetic processes were obtained from the absorbance changes in specific bands. This in situ sampling technique demonstrates a new area of Fourier transform infrared (FTIR) spectroscopy for following the formation of polymeric bonds. © 1995 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070560604
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Raman spectrum of matrix isolated hydrogen sulfide (1974)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 2 (1974), S. 209-215 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra of H2S isolated in N2 at M/A ratios of 30, 50, 100, and 300 and in Ar at M/A ratios of 50, 100, 300, and 1000 have been measured in the S-H stretching region. The spectral results confirm the infrared measurements on H2S isolated in N2; however, the symmetric stretching vibration, v1, of the H2S monomer is at about the same frequency, 2619 cm-1, in the spectra of Ar matrices. This disagrees with previous infrared measurements of Ar matrices. Raman bands in the spectra of both N2 and Ar matrices due to a dimer of H2S are consistent with those observed in the infrared spectrum of N2 matrices, and they support a donor-acceptor model for the dimer.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250020211
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Raman spectra of gaseous and matrix-isolated SO3 (1975)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 3 (1975), S. 387-390 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250030409
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    Paper (German National Licenses)
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