ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The effects of increasing concentartions of methylmercuric hydroxide (CH3HgOH) on the rate of sedimentation, S0, and intrinsic viscosity, [η], of T7 DNA have been studied at 20°C in 0.005, 0.05, and 0.5M Na2SO4, respectively, whereby each salt solvent conatined, in addition, 0.005M sodium borate, pH 9.18, as a buffer. Both S0 and [η] are independent of organomercurial concentration as long as DNA remains native. Denaturation, brought about by the complexing of CH3HgOH with the polymer, produces large changes in S0 as wll as [η]. The sedimentation coefficient increases strongly with increasing oragnomercurial concentration once strand separation has occured. Experimental difficulties prevented measuring of [η] in the posttransition region. The data on S0 have been used, in combination with available information on the so-called density increment (∂ρ/∂c2)0μ, to obtain information on the frictional properties of single-stranded and methylmercurated T7 DNA. The frictional coefficient, defined as f′2 = M2(∂p/∂c2)0μ/S0ηNA, where M2 is the molecular wieght of T7 DNA, c2 is the concentration of DNA in g/ml of solution, ηr the realtive viscosity of the salt solvents, and NA is Avogadro's number, was evaluated for all three salt media as a function of organomercurial concentration. f′2 of native T7 DNA was found not to be sensitive to changes in ionic strngth; but f′2 of single-stranded and methylmercurated T7 DNA varied strongly with salt concentration. Since f′2 of single-stranded T7 DNA was barely affected by organomercurial concentration at a given ionic strength, it is concluded that the dramatic variations of S0 with pM (pM ≡ -log[CH3HgOH]) observed in the posttransition zone reflect only changes in the thermodynamic interactions (“preferential interactions”) existing between DNA and the vatious other solution components, but not changes in the coil dimensions of the polymer.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1978.360170306
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