ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A method of calculation to determine the profile in density-gradient sedimentation of bromodeoxyuridine-substituted DNA lengths prior to sonication is developed. The method may be summarized as follows: (1) A description of a formulation of random cleavage of a substituted zone i in fragments of length m; (2) generalization and application of this formulation to all substituted zone lenghts simultaneously present in a given DNA extract, such generalization being performed by means of a matrix calculation; (3) adjustment of this formulation for the limits of the experimental method, i.e., number of gradient fractions which can be recovered, and for the evolution of the DNA fragment lengths which drop in the course of successive sonications; (4) simulation of the radioactive profiles. By this method it is possible to obtain certain indications concerning the types of initial (i.e., presonication) distributions that are compatible with observed profiles and to test the validity of assumptions relating to the initial distribution. This method has been applied in analyzing DNA synthesis in lymphocytes stimulated by Concanavalin and allowed us to determine the significance of the discontinuties appearing in the elongation with a suboptimal stimulating dose. The proposed method could also be applied to the analysis of all random populations of mixed sequences.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1978.360171209
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