ISSN:
1040-7685
Keywords:
capillary electrophoresis
;
polyamines
;
cylindrical lens
;
fluorescence detection
;
fluorescamine derivatives
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Free polyamines in rat tissues were determined by high performance capillary electrophoresis using fluorescence detection. After precipitation of proteins with perchloric acid, the sample solution was derivatized with fluorescamine using a microscale procedure. Ethylenediamine was added to the medium to avoid adsorption of polyamines onto the capillary wall. An internal standard of 6-amino-n-caproic acid a was used. To improve detector sensitivity, the fluorescence signal was collected with a cylindrical lens. The minimum detectable amount and concentration of the sample for the present method were around 200 fmol and 10 nmol mL-1, respectively.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mcs.1220020106
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