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  • 1
    Electronic Resource
    Electronic Resource
    Detection of low-molecular-weight heparin oligosaccharides (Fragmin™) using surface plasmon resonance (1998)
    Chicester [u.a.] : Wiley-Blackwell
    Journal of Molecular Recognition 11 (1998), S. 191-193 
    Details
    ISSN: 0952-3499
    Keywords: Surface plasmon resonance ; low-molecular-weight heparin ; oligosaccharides ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: During the last decades there has been a growing realization of the central biological role that oligosaccharides and oligosaccharide-protein interactions play. One of the most striking examples is the use of heparin and low-molecular-weight heparin oligosaccharides (Fragmin™) to modify blood coagulation. Several monoclonal antibodies directed against glycosaminoglycan structures have been produced. However, their clinical use is limited by the difficulty of detection systems for oligosaccharides. In the present study we used a monoclonal antibody directed against heparin oligosaccharides prepared by partial nitrous acid deamination of heparin. Using a biosensor (BIAcore™), purified antibody was immobilized on sensor surfaces and binding of oligosaccharide was measured by surface plasmon resonance. Using this technique, it was possible to quantitate low-molecular-weight heparin oligosaccharides in nanomolar concentrations. Copyright © 1998 John Wiley & Sons, Ltd.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Quantitation of a urinary tetrasaccharide by gas chromatography and mass spectrometryAbbreviations: GSD = glycogen storage disease; DMD = Duchenne muscular dystrophy; (Glc)4 = glucose-containing tetrasaccharide α-D-Glc-(1 → 6)-α-D-Glc-(1 → 4)-D-Glc-(1 → 4)-D-Glc; (IM)4 = isomaltotetraose. (1976)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 3 (1976), S. 51-54 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A gas chromatographic mass spectrometric method has been developed for the rapid determination of a urinary tetrasaccharide (α-D-Glc-(1 → 6)-α-D-Glc-(1 → 4)-α-D-Glc-(1 → 4)-D-Glc). The urine sample is first fractionated by gel chromatography. An appropriate internal standard is added to the pooled tri-pentasaccharide fraction. Which is then reduced, methylated and fractionated by g.c. The identification of the tetrasaccharide derivative is based on the g.c. relative retention time and the mass spectrum of the reduced permethylated tetrasccharide derivative is based on the g.c. relative retention time and the mass spectrum of the reduced permethylated tetrasaccharide. The normal excretion rate was in the range of 0.1-2.5 mg per 24 hours. Greatly increased amounts (9.4-89.6 mg 24 h-1) were found in the urine of patients with glycogen storage disease type II and type III and in one patient with unclassified muscular disease. A moderate increase (3.6-8.6 mg 24 h-1) was observed in one patient with glycogen storage disease type VI. in two patients with Duchenne muscular dystrophy and in two other patients with unclassified muscular disease.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200030202
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  • 3
    Electronic Resource
    Electronic Resource
    Use of weak monoclonal antibodies for affinity chromatographyPart of this work has been published in: Ohlson, S., Bergström, M., Påhlsson, P. and Lundblad A. (1997). Use of monoclonal antibodies for weak affinity chromatography. J. Chromatogr. A 758, 199-208. (1998)
    Chicester [u.a.] : Wiley-Blackwell
    Journal of Molecular Recognition 11 (1998), S. 110-113 
    Details
    ISSN: 0952-3499
    Keywords: weak affinity chromatography (WAC) ; monoclonal antibodies ; carbohydrate antigen ; perfusion chromatography ; high-performance liquid affinity chromatography (HPLAC) ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: When using weakly interacting ligands in affinity chromatography, it is possible to take advantage of a true chromatographic process in the separation, as compared with traditional affinity chromatography which is rather an on/off process. In this work, weak monoclonal antibodies were immobilized on a silica and a perfusion-type support (POROS AL) and used for high-performance liquid affinity chromatography (HPLAC). Similar carbohydrate antigens were separated under isocratic conditions according to their weak interaction with the immobilized monoclonal antibody. The affinity of the antibodies was adjusted with temperature and pH to modify the separation. The productivity of the chromatographic system was increased with the immobilized perfusion support but at the expense of loss of plate numbers. This study clearly demonstrates the potential of weak affinity biological interactions as a basis for chromatographic separation. Copyright © 1998 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 4
    Electronic Resource
    Electronic Resource
    Determination of a mannose-containing trisaccharide in the urine of patients and heterozygotes of mannosidosis (1975)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 2 (1975), S. 285-287 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method employing gas-liquid chromatography and combined gas-liquid chromatography mass spectrometry was developed for the quantitative determination of α-D-mannopyranoside-(1 → 3)-β-D-mannopyranoside-(1 → 4)-2-acetamido-2-deoxy-D-glucose in urines. The concentration of this trisaccharide varied between 123 and 469 mg per litre of urine in the patients with mannosidosis but was not detected in twenty normals and two heterozygotes.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200020602
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    Paper (German National Licenses)
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