ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Curie-point pyrolysis mass spectrometry was performed on three bacterial isolates obtained from infected human urine using a novel, direct isolation method. Bacterial samples were analysed directly after isolation, as well as after free growth in broth and after exposure to solutions containing penicillin and gentamicin. The spectra of bacteria directly from urine showed no detectable contamination from urinary constituents. Discriminant analysis indicated genetic strain differences to be greater than the combined variances due to sample preparation or the growth phase. Characteristic biochemical changes related to growth or non-growth were detectable after only 2 h of incubation. The potential usefulness of pyrolysis mass spectrometry techniques for rapid susceptibility testing is discussed.
Additional Material:
10 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200130604
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