ISSN:
1432-1335
Keywords:
Proliferation
;
Migration
;
ras
;
Collagen matrix
;
Flavonoids
;
7-Hydroxycoumarin
;
Genistein
;
Chemoprevention
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The effects of 7-hydroxycoumarin, genistein and quercetin on tworas-oncogene-driven tumour cells (rat breast adenocarcinoma and human bladder carcinoma) were investigated using cellular (proliferation and migration) and molecular targets (p21 ras GTPase activity and intracellular amount of p21 ras protein). All three compounds inhibited the growth of both cell lines. Genistein was the most effective substance. Further-more, 7-hydroxycoumarin and genistein affected the motile machinery of both cell lines because major fractions of the cells were slowed down or stopped locomotion. The phorbol ester, phorbol 12-myristate 13-acetate (PMA), a well-known tumour promoter, increased the locomotion behaviour of the cells; the time of migration, the velocity and the distance of migration increased under the control of PMA. 7-Hydroxycoumarin decreased the relative amount of intracellular p21 ras , and concomitantly a PMA-induced decrease of p21 ras , GTPase activity could be partially antagonized by 7-hydroxycoumarin. Because of the low toxicity and the mode of action evaluated, it is likely that the best role for these substances may be adjuvant therapy of some malignancies following surgery. Profiles directed to migration and proliferation inhibition make these drugs exceptional candidates for chemopreventive strategies in tumours diagnosed as having increasedras oncogene levels.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01220800
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