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  • 1
    ISSN: 1432-1335
    Keywords: Gynecological tumors ; Cellular heterogeneity in culture ; Chemosensitivity ; Proliferation assay ; Reproducibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Procedures for the in vitro determination of the drug-induced inhibition of mammary and ovarian carcinoma cell growth were established. In monolayer cultures derived from advanced tumors, separation of epithelial carcinoma cells from concomitant cells of fibroblast-like or mesothelial appearance was achieved by differential trypsinization. The carcinoma cell character of the stock cultures was verified by chromosome analyses showing a high degree of aneuploidy for the epitheloid cell lines and euploidy for cells of apparently mesenchymal origin. When cultured carcinoma cells were injected in nu/nu mice, the tissue and cell cultures obtained from the heterotransplantation tumors closely resembled the original tumors and cell cultures in morphology, karyotype, and expression of tumor markers. The action of carcinostatic drugs in the logarithmic phase of the carcinoma cell proliferation was tested by kinetics experiments in multiple experimental cultures. In cell proliferation assays based on cell counts the 50% inhibition dose (ID50) of the drug effects was determined from the dose-response curves. Comparison of the ID50s revealed highly differential effectiveness of the drugs examined. The inhibitory effects were reproducible, rendering the procedures used suitable for testing the chemosensitivity of newly explanted gynecological carcinoma cells by proliferation assays.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 349 (1979), S. 582-583 
    ISSN: 1435-2451
    Keywords: Liver tumors ; Oral contraceptives ; Diagnosis ; Therapy ; Benigne Lebertumoren ; Kontrazeption ; Diagnose ; Therapie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Vom 1.1.1976–1.10.1978 wurden 8 Frauen, die jahrelang Kontrazeptiva eingenommen hatten, mit gutartigen Lebertumoren behandelt, in allen Fällen lautete die histologische Diagnose: fokale noduläre Hyperplasie. Bei 6 Patientinnen war der Tumor uni-, bei 2 bilokulär. Als diagnostische Verfahren bewährten sich in der Reihenfolge Computertomo-, Sono-, Szinti-und Angiographie. Eine Artdiagnose war nur mit der Laparoskopie und Biopsie aus dem Tumor und dem gesunden Lebergewebe möglich. - Da diese Tumoren bluten und selten maligne entarten können, wurden sie durch Enucleation oder Resektion entfernt. Zwei auf den Lebervenen lokalisierte Zweittumoren wurden belassen. Die Letalität betrug 0 %. Nach 1-2jähriger Beobachtungszeit haben sich die belassenen Geschwülste nach Absetzen der Kontrazeptiva nicht verkleinert. Die chirurgische Entfernung dieser Tumoren wird wegen ihrer Persistenz und ihrer Komplikationsmöglichkeiten als Behandlungsverfahren der Wahl empfohlen.
    Notes: Summary From 1.1. 1976-1.10.1978 8 women taking oral contraceptives for many years were admitted with benign hepatic tumors. The histological diagnosis in all cases was focal nodular hyperplasia. In 6 women the tumor was single, in 2 women multicentric. Diagnosis by computer tomography, sonography, liver scanning and angiography. A specific diagnosis is only possible by laparoscopy with biopsy of the tumour and the normal liver tissue. Because of the danger of rupture and intra-abdominal hemorrhage and a rare trend to malignancy, enucleation or resection of these tumours was performed; two of the multicentric hepatomas localized at the hepatic veins remained in place. Mortality was 0 %. After cessation of oral contraceptives the remaining tumors did not change in size during one to two years. Enucleation or resection is recommended treatment of choice for benign hepatic tumors.
    Type of Medium: Electronic Resource
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