ISSN:
1432-072X
Keywords:
“Slime” variant
;
Neurospora
;
Chitosomes
;
Chitin synthetase
;
Secretory vesicles
;
Invertase
;
Phosphatase
;
Membranes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cells from the “slime” variant of Neurospora crassa were broken in isotonic conditions by use of triethanolamine buffer plus EDTA. After removal of large membranous structures by low-speed centrifugation, chitosomes and secretory vesicles were separated by means of gel filtration, precipitation of membranous contaminants with Concanavalin A, and centrifugation in sucrose or glycerol gradients. Polypeptidic composition of fractions enriched in secretory vesicles or chitosomes was found to be distinct. By these criteria we concluded that chitosomes and secretory vesicles represent different populations of microvesicles. Both microvesicular populations appeared free of endoplasmic reticulum and vacuolar contaminants as demonstrated by determination of appropriate enzymatic markers.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425082
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