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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural research on cell wall regeneration by cultured pollen protoplasts of Lilium longiflorum (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 36-45 
    Details
    ISSN: 1432-2145
    Keywords: Pollen protoplast ; Pollen tube ; Lilium longiflorum ; Cell wall regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts from pollen grains of Lilium longiflorum regenerate amorphous cellulosic cell walls in culture, during which some precursors of cellulose are polymerized, thus producing progressively harder cellulosic cell walls as the period of culture continues. It is presumed that the components of the cell wall regenerated during 1 week in culture differ from those of the intine of the pollen grain wall. The regenerated cell wall is formed by means of large smooth vesicles; in addition, numerous coated vesicles and pits aid in wall regeneration. The pollen tube that germinates from the 8-day-old cultured protoplast has numerous Golgi bodies and many vesicles which build the pollen tube wall. The tube wall has two layers just like a normal pollen tube wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227021
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Epifluorescent microscopic studies on the mechanism of preferential destruction of chloroplast nucleoids of male origin in young zygotes ofChlamydomonas reinhardtii (1985)
    Springer
    Protoplasma 125 (1985), S. 43-52 
    Details
    ISSN: 1615-6102
    Keywords: Chlamydomonas reinhardtii ; Chloroplast nucleoidal destruction ; RNA synthesis ; UV interference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The studies on the kinetics of nucleoid destruction reported here showed that destruction of chloroplast nucleoids (ct nucleoids) of male origin began to occur at about 30 minutes after mixing of male (mt−) and female (mt+) gametes. The timing of initiation of the destruction differed among zygotes but usually occurred during 50–120 minutes after mixing. About 10 minutes was required for complete digestion of the ct nucleoids. UV irradiation on young zygotes or addition of an RNA-synthesis inhibitor, actinomycin D, to the incubation medium during the first 0–30 minutes after mixing almost completely inhibited the incorporation of3H uridine into the cell nuclei and the preferential destruction without inhibiting cell nuclear fusion. These results suggest that soon after mating,de novo RNA synthesis is concerned for the preferential destruction of ct nucleoids. To determine in which of the two cell nuclei in the zygotes the RNA is synthesized, each gamete (mt−, mt+) was irradiated with UV and mated with unirradiated gametes of opposite mating type. This treatment of the male gametes had no effect on the incorporation of3H uridine into cell nuclei and the preferential destruction of ct nucleoids but UV irradiation of female gametes almost completely inhibited the incorporation of3H uridine into cell nuclei and the preferential destruction of ct nucleoids. Similar phenomena occurred in other crosses. The UV effect was photoreactivated in about 50% by white light, suggesting that the UV target is DNA. Thus, RNA synthesized in the cell nucleus of female origin soon after mating may be responsible for the preferential destruction of ct nucleoids of male origin
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01297349
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Chloroplast nucleoids in large number and large DNA amount with regard to maternal inheritance inChlamydomonas reinhardtii (1996)
    Springer
    Protoplasma 194 (1996), S. 11-17 
    Details
    ISSN: 1615-6102
    Keywords: Chloroplast nucleoid number ; Chloroplast DNA amount ; Preferential digestion ; Maternal inheritance ; Chlamydomonas reinhardtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We studied the maternal chloroplast inheritance ofChlamydomonas reinhardtii by epifluorescence microscopy after staining with DNA specific fluorochrome DAPI and by genetic methods, using wild type cells and cells containing previously isolated mutation of cond-1 and cond-2. Wild type cells contained about 7 chloroplast (cp) nucleoids, while mutants, cond-1(+) and cond-2(+), contained about 14 and 23 cp nucleoids, respectively, after one week culture on agar plates. The total cpDNA contents were almost proportional to the numbers of cp nucleoids. When cells containing cond-1 or cond-2 mutation were used as a parental source to cross with wild type cells of the other parent, preferential digestion of cp nucleoids from male parent (mt−) origin occurred in the zygotes, although the frequencies of the digestion were slightly lower than that in the zygotes from the cross between wild type cells. Western blot analysis of the protein ofzyslB gene, which has been found related to preferential digestion of mt− origin cp-nucleoids DNA, showed that a high amount of this protein was detected with the initiation of preferential digestion of mt− cp nucleoids and disappeared with the completion of the digestion. Cp genetic markers for antibiotic resistance were maternally inherited in all crosses. These results showed that although the preferential digestion of cp nucleoids consisting of large number and large cpDNA amount requires a slightly longer period to complete, this high ploidy of the cp nucleoids does not disturb maternal inheritance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01273163
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of callose in the germinated pollen ofCamellia japonica (1996)
    Springer
    Protoplasma 194 (1996), S. 133-139 
    Details
    ISSN: 1615-6102
    Keywords: Camellia japonica ; Callose ; Pollen tube ; Callose plug ; Golgi vesicle ; Immuno-localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A polyclonal antibody against β-1,3-glucan, callose, extracted from the pollen tube wall ofCamellia japonica was raised in mice and, using it as a probe, the localization of callose in the germinated pollen was studied. By confocal laser scanning microscopy, callose was found in the tip region of the pollen tube and the tube wall; the immuno-fluorescence in the tube wall was less toward the base of the tube. In contrast, the tip region did not fluoresce although the whole of the tube wall did strongly with aniline blue, the specific dye for callose. Immuno-electron microscopy showed that callose was also found in Golgi vesicles which concentrated in the tip region of the pollen tube, the inner layer of the tube wall, callose plugs, and Golgi vesicles in the pollen grain. Immuno-gold labeling was often detected on the fibrous structures in Golgi vesicles and callose plugs. Based on these results, the participation of Golgi vesicles in the formation of the tube wall and callose plugs was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01882021
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of myrmicacin (β-hydroxydecanoic acid) on protoplasmic movement and ultrastructure ofCamellia japonica pollen (1981)
    Springer
    Protoplasma 105 (1981), S. 341-345 
    Details
    ISSN: 1615-6102
    Keywords: Golgi-vesicles ; Myrmicacin ; Pollen tube ; Protoplasmic movement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary While tube elongation of growing pollen ofCamellia japonica was stopped by treatment with 50–100 ppm of myrmicacin, protoplasmic movement in the pollen tube still continued. However, higher concentration (200 ppm) of the inhibitor arrested the movement. The vesicles containing membrane substances disappeared at the tip of the tube of the growth-inhibited pollen. Removal of the inhibitor resulted in the reappearance of the vesicles at the tip region and tube elongation was restored.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279231
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    Paper (German National Licenses)
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