ISSN:
1432-2013
Keywords:
Ischaemia
;
Reperfusion
;
Chloride conductance
;
Potassium conductance
;
Action potential
;
ATP
;
Rat skeletal muscle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Electrical parameters of extensor digitorum longus (EDL) muscles and their contralaterals were measured “in vitro” at 30°C by a computerized two intracellular microelectrode technique after ischaemia and postischaemic reflow. In some muscles the adenosine triphosphate (ATP) levels are also measured. Ischaemia led to a 39% reduction of Cl− conductance (G Cl), whereas reperfusion increase G Cl by 18% with respect to contralateral control muscles. Ischaemia and reperfusion increased K+ conductance (G K) by 21% and 68%, respectively; this increased was reversed by 50 μM glybenclamide, suggesting an involvement of ATP-sensitive K+ channels. A statistically significant hyperpolarization and increase in excitability was observed after ischaemia, whereas after the reflow period the fibres were depolarized and less excitable. Ischaemia and reperfusion lowered the intracellular ATP content by 18% and 64%, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00374669
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