ISSN:
1612-1112
Keywords:
RP-HPLC
;
Mobile phase
;
Optimization
;
Gradient elution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Quantitative analysis of more than ten compounds in a sample generally requires complex mobile phases to optimize the separation of the analytes by gradient elution reversed-phase HPLC. For this purpose, CHEOPS, a software package has been developed from the fully stochastic Computer Chromatogram Simulation Method. Calculation principles and optimization criteria are described. Experimental validation is presented with amino acids and steroids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02324713
Permalink