ISSN:
1432-203X
Keywords:
Cichorium intybus
;
Agrobacterium tumefaciens
;
Chlorsulfuron resistance
;
Kanamycin resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Leaf discs of C. intybus were inoculated with an Agrobacterium tumefaciens strain harboring a neomycin phosphotransferase (neo) gene for kanamycin resistance and a mutant acetolactate synthase gene (csr1-1) from Arabidopsis thaliana conferring resistance to sulfonylurea herbicides. A regeneration medium was optimized which permitted an efficient shoot regeneration from leaf discs. Transgenic shoots were selected on rooting medium containing 100 mg/l kanamycin sulfate. Integration of the csr1-1 gene into genomic DNA of kanamycin resistant chicory plants was confirmed by Southern blot hybridizations. Analysis of the selfed progenies (S1 and S2) of two independent transformed clones showed that kanamycin and chlorsulfuron resistances were inherited as dominant Mendelian traits. The method described here for producing transformed plants will allow new opportunities for chicory breeding.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00235074
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