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  • 1
    Electronic Resource
    Electronic Resource
    Influence of cholesterol on gramicidin-induced H"I"I phase formation in phosphatidylcholine model membranes
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 939 (1988), S. 79-88 
    Details
    ISSN: 0005-2736
    Keywords: Cholesterol ; Differential scanning calorimetry ; Gramicidin ; Hexagonal H"I"I phase ; Model membrane ; NMR, ^2H- ; NMR, ^3^1P- ; Phosphatidylcholine ; Small angle X-ray diffraction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(88)90049-1
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  • 2
    Electronic Resource
    Electronic Resource
    Comparative ^2H- and ^3^1P-NMR study on the properties of palmitoyllysophosphatidylcholine in bilayers with gramicidin, cholesterol and dipalmitoylphosphatidylcholine
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 854 (1986), S. 133-142 
    Details
    ISSN: 0005-2736
    Keywords: Bilayer structure ; Cholesterol ; Gramicidin ; Lysophosphatidylcholine ; NMR ; Order profile
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(86)90073-8
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  • 3
    Electronic Resource
    Electronic Resource
    Distinct mechanisms of interferon-gamma and tumor necrosis factor-alpha action in oncogene-transformed mouse fibroblasts (1988)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 38 (1988), S. 205-212 
    Details
    ISSN: 0730-2312
    Keywords: cytokines ; gene expression ; retroviral promoters ; antitumoral activities ; phenotypical reversion ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The potential mechanisms of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha action on tumor cells have been investigated in a model of mouse fibroblasts transformed by distinct retroviral vectors carrying the v-mos, c-myc, and v-Ha-ras oncogene, respectively. Treatment with both cytokines not only caused growth inhibition of v-mos- and c-myc-transformants, but also a reversion of transformation-induced suppression of major histocompatibility complex (MHC) class I antigen expression in all transformed cell lines. The phenotypical reversion of transformants was preceded by a selective modulation of LTR-controlled oncogene expression. TNF-alpha primarily affected stability of oncogene-specific RNAs without influencing the activity of retroviral promoters. In contrast, IFN-gamma was effective at the transcriptional level, apparently due to inhibition of LTR activity as revealed from reduced CAT activity in IFN-gamma-treated LTR-CAT transformants. This IFN-gamma-mediated down-regulation of retroviral promoter activity seemed to be selective for Moloney-virus-derived promoters, since the activity of other viral and cellular promoters was not suppressed by IFN-gamma.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240380308
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  • 4
    Electronic Resource
    Electronic Resource
    Composition and morphology of lipid droplets from oviduct epithelial cells (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 466-474 
    Details
    ISSN: 0003-276X
    Keywords: Bovine ; Cholesterol ; Phospholipid ; Epithelia ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: This study was undertaken to determine the composition and morphology of lipid droplets in situ and isolated from oviductal epithelial cells and oviductal fluid. Oviductal epithelial cells were harvested enzymatically from oviducts of cows in either the luteal or the follicular stages of the ovarian cycle. Lipid droplets were isolated from cellular homogenates and characterized biochemically using thin layer chromatography. The morphology of lipid droplets in oviductal epithelial cells and in fractions isolated by ultracentrifugation from cellular homogenates was examined by electron microscopy. Lipid droplets isolated from oviduct epithelial cells varied in composition with the ovarian cycle and the oviductal region. There was more total lipid in droplets isolated from cows in the luteal than follicular phase of the ovarian cycle. Most of this difference was due to large amounts of esterified cholesterol present in the samples from luteal-stage animals. The most esterified cholesterol was found in droplets isolated from the oviductal isthmus of luteal cows. Droplets similar in lipid composition to those isolated from epithelial cells were found in oviductal fluid. Four distinct types of lipid inclusions were evident in electron micrographs of oviductal epithelia and characterized as osmiophilic droplets, lipofuscin-like clusters, lamellar structures, and composite bodies. All of the lipid inclusions were found in droplet isolates except for the extracted lipid portion of the composite body. The presence and diversity of oviduct epithelial lipid inclusions suggest that the oviductal epithelium may be very active in lipid metabolism, particularly cholesterol dynamics. © 1993 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092370405
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  • 5
    Electronic Resource
    Electronic Resource
    In vitro and in vivo association of an oviduct estrus-associated protein with bovine zona pellucida (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 29 (1991), S. 77-84 
    Details
    ISSN: 1040-452X
    Keywords: Immunofluorescence techniques ; Polyclonal antiserum ; Oviduct fluid ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The interaction between the bovine egg zona pellucida and a 97 kDa estrus-associated protein produced by the oviduct was examined in vitro and in vivo. In vitro matured bovine eggs were incubated with oviduct fluid recovered throughout the estrous cycle from separate indwelling cannulae placed in the ampulla and isthmus of the same oviduct. Immunofluorescence techniques and a polyclonal antiserum against the 97 kDa protein were used to localize this protein on washed eggs previously incubated with oviduct fluid. Intensity and distribution of immunofluorescence varied with stage of cycle and to a lesser degree with region of oviduct from which the oviduct fluid was obtained. The most intense fluorescence was observed on the zonae pellucidae of eggs incubated with oviduct fluid pooled from days near estrus and ovulation compared to fluid pooled from luteal stage days. The immunofluorescence of isthmus-derived oviduct fluid was more intense than was ampulla-derived oviduct fluid collected near estrus. The zonae pellucidae of 7-day-old embryos flushed from the uterus displayed immunofluorescence comparable to that observed on the zonae pellucidae of eggs incubated in vitro with peri-estrus oviduct fluid. No immunofluorescence was observed associated with the perivitelline space, egg cytoplasm, or blastomeres. The apparent uptake of a 97 kDa estrus-associated protein by the zonae pellucidae of eggs in vitro and embryos in vivo may indicate that this protein functions in fertilization and/or early embryo development.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080290112
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  • 6
    Electronic Resource
    Electronic Resource
    Adsorption of oviductal fluid proteins by the bovine sperm membrane during in vitro capacitation (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 33 (1992), S. 313-323 
    Details
    ISSN: 1040-452X
    Keywords: Oviduct ; Sperm membrane ; Proteins ; Capacitation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: 125I-labeled oviductal fluid (ODF) proteins and antiserum to ODF were used to determine whether ODF proteins associate with the sperm membrane during in vitro capacitation. Luteal and nonluteal pools of ODF were obtained from oviduct catheters during the estrous cycle. Washed sperm (50 × 106 sperm/ml) were incubated up to 4 h in a protein-free modified Tyrode's medium (MTM), or MTM supplemented with 40% ODF, or 0.5 ng 125I-labeled ODF proteins. Solubilized sperm membrane proteins and incubatin media containing ODF proteins were separated by gel electrophoresis. Membranes isolated from bovine sperm previously incubated with ODF, absorbed five 125I-proteins: A doublet at 85-95 kDa, and others at 24, 34, 53, and 66 kDa. The amount of 66 kDA 125I-protein associated with the sperm decreased during the incubation, whereas the amount of 85 to 95-kDa protein did not. Western blot analyses also detected the presence of ODF proteins (53, 66, 85-95, and 116 kDa) in solubilized membranes from sperm incubated in ODF. The 85 to 95-kDa protein in ODF decreased in ODF. The 85 to 95kDa protein in ODF decreased in apparent molecular weight by 5 kDa when associated with the sperm membrane. At 53 kDa ODF proteins which associated with sperm were transformed from two to three separate proteins. These studies indicate that the surface of sperm is modified by adsorption of ODF proteins ot the membrane during in vitro capacitation. (c) 1992 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080330313
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  • 7
    Electronic Resource
    Electronic Resource
    Role of the extracellular matrix in tissue-specific gene expression in the sea urchin embryo (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 29 (1991), S. 220-226 
    Details
    ISSN: 1040-452X
    Keywords: ECM ; Differentiation ; Strongylocentrotus purpuratus ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of β-aminoproprionitrile fumarate (BAPN), which disrupts collagen deposition, and β-D-xyloside, which disrupts proteoglycan metabolism. The markers examined included accumulation of primary mesenchyme-specific mRNA (SM 50); and aboral ectoderm-specific mRNA (Spec 1); and a gut-specific enzyme, alkaline phosphatase. Treatment with BAPN or β-D-xyloside results in developmental arrest at the mesenchyme blastula stage. Although spicule formation is inhibited, the accumulation of SM 50 transcripts and the synthesis of most of the prominent spicule matrix proteins is similar to that of control embryos. Spec 1 mRNA, in contrast, while accumulating to a significant extent when collagen and proteoglycan metabolism is disrupted, does accumulate to a level somewhat lower than that seen in control embryos. Additionally, the postgastrula rise in gut-specific alkaline phosphatase is reversibly inhibited by BAPN and xyloside treatment. These results demonstrate a differential effect of the ECM on expression of tissue-specific molecular markers.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080290303
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  • 8
    Electronic Resource
    Electronic Resource
    Increased uptake of thymidine in the activation of sea urchin eggs. III. Effects of aphidicolin (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 118 (1984), S. 27-33 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Uptake and phosphorylation of externally supplied [3H]-thymidine are fully stimulated in fertilized sea urchin eggs exposed to 5.0 μg/ml aphidicolin. As in untreated controls, the rate of uptake in aphidicolin-treated eggs increases greater than 50-fold shortly after fertilization, and greater than 85% of the transported thymidine is immediately phosphorylated to the triphosphate. The intracellular levels of [3H]-thymidine triphosphate (3H-dTTP) resulting from an external supply of [3H]-thymidine is therefore equal in aphidicolin-treated and untreated fertilized eggs. Under the same experimental conditions, the incorporation of externally supplied [3H]-thymidine into newly synthesized DNA of fertilized eggs is 90% inhibited by exposure to aphidicolin. The full availability of 3H-dTTP in these eggs further suggests that aphidicolin inhibits specifically at the level of DNA synthesis. This inhibitory effect is proportional to the concentration of aphidicolin between 0 and 5.0 μg/ml. In the continuous presence of 5.0 μg/ml aphidicolin, fertilized eggs fail to undergo mitotic chromosome condensation, nuclear envelope breakdown, and cytokinesis, suggesting a dependent link between these processes and the completion of nuclear DNA synthesis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041180107
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