ISSN:
1612-1112
Keywords:
Supercritical fluid chromatography
;
Supercritical fluid extraction
;
Edible fats
;
Fatty acids
;
Cholesterol
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Geometric isomers of fatty acids were separated by open tubular columns in SFC. The concentration of the analytes varied between 9 and 16 μg mL−1. Quantification of triglycerides with repeatability better than 20% were obtained in a home made SFE-SFC unit. A four point calibration curve for both trimyristin and tripalmitin was developed with correlation coefficients of 0.998 and 0.9998, respectively. The limit of quantification was approximately 3 ng for both components. Supercritical CO2 as extraction solvent in matrix containing lipids increased the recovery of cholesterol by a factor of three. Using immobilized lipase in on-line SFE-SFC quantification of cholesterol in spray dried egg yolk was possible.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02466317
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