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  • Development  (3)
  • Collecting duct  (2)
  • Tissue culture  (2)
  • Chromatographie, HPLC/Chromatographie, Gas  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Ionic conductances of cultured principal cell epithelium of renal collecting duct (1988)
    Springer
    Pflügers Archiv 412 (1988), S. 434-441 
    Details
    ISSN: 1432-2013
    Keywords: Collecting duct ; Principal cell ; Tissue culture ; Chloride conductivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ionic conductive properties were studied of epithelia of collecting duct principal cells which had been grown in primary tissue culture from renal cortex/capsule explants. When pretreated with aldosterone (10−6 mol/l) and bathed on either surface with isotonic HCO 3 − -free Ringer's solution, the transepithelial voltage,V te, varied between −21 and −72 mV (apical surface negative) while the transepithelial resistance,R te, ranged from 0.4 to 1.5 kΩcm2. By 10:1 step-changes in Na+ concentration the apical cell membrane was shown to have a high conductivity for sodium, inhibitable by amiloride, 10−6 mol/l. However, contrary to observations in natural collecting duct under control conditions, amiloride never reversed the polarity ofV te even at 10−4 mol/l. Both the apical and the basolateral cell membranes were conductive for potassium and both conductivities were inhibitable by Ba2+ (5 mmol/l). 10:1 reduction of apical Cl− concentration strongly hyperpolarizedV te with a monophasic time course suggesting the presence of a paracellular shunt conductance for Cl−. In addition there may be a small Cl− conductance present in the apical cell membrane since apical application of the chloride channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPAB) at 10−7 mol/l produced a minute but significant hyperpolarization. On the other hand, 10:1 reduction of basolateral Cl− concentration caused a biphasic change inV te (initial depolarization, followed by repolarization) which indicates the presence of a large Cl− conductance in the basolateral cell membrane. The latter was not inhibitable by 10−7 mol/l NPPAB. Higher concentrations of this and of an other Cl− channel blocker produced non-specific effects. In conclusion, our studies of a pure principal cell epithelium confirm findings described for the intact cortical collecting duct and add new information concerning chloride conductivity and related blocking agents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01907564
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  • 2
    Electronic Resource
    Electronic Resource
    Culture of embryonic renal collecting duct epithelia in a gradient container (1997)
    Springer
    Pediatric nephrology 11 (1997), S. 140-147 
    Details
    ISSN: 1432-198X
    Keywords: Key words: Collecting duct ; Development ; Perfusion culture ; Gradient ; Principal and intercalated cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. During organogenesis the ampullar epithelium of the renal collecting duct acts as an inducer which generates all of the nephron anlagen. As development proceeds, one part of the collecting duct cells in the ampullar tip retain their inducer capability, while others develop into the functional epithelium consisting of principal and intercalated (IC) cells. The events leading from the embryonic inducer to the mature tissue are unknown. We investigated the maturation of embryonic collecting duct epithelium derived from neonatal rabbit kidney under in vitro conditions. To prevent dedifferentiation the epithelia were cultured on kidney-specific support material within a tissue carrier. Apical and basal compartments of the epithelia were simulated in a gradient culture container. The two sides of the epithelium were each constantly perfused with a different medium. During the 14-day incubation the tissue was not subcultured. The development of collecting duct cell features was investigated with morphological and immunohistochemical methods. Both light and electron microscopy revealed morphologically intact epithelia following gradient culture. The polarized cells rested on a uniformly developed basement membrane. The continuous application of aldosterone during the culture modulated the development of collecting duct cell characteristics. Both basal and luminal administration of aldosterone initiated differentiation in the embryonic epithelia. Using the sodium (Na) channel blocker amiloride, it was demonstrated that Na channels are involved in the differentiation of the IC cell phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004670050245
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  • 3
    Electronic Resource
    Electronic Resource
    Electrical properties of renal collecting duct principal cell epithelium in tissue culture (1986)
    Springer
    Pflügers Archiv 406 (1986), S. 380-386 
    Details
    ISSN: 1432-2013
    Keywords: Collecting duct ; Principal cell ; Tissue culture ; Aldosterone ; Amiloride ; Sodium transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Whereas collecting duct epithelium in vivo is composed of principal and intercalated cells, we grew a pure principal cell epithelium using a new technique involving tissue culture. These principal cells were derived from collecting duct anlagen of newborn rabbits. We investigated the electrical properties of such epithelia in a newly designed lucite double-chamber with an inner opening of 0.08 cm2. Our observations were: 1) mean transepithelial resistanceR te was 0.83±0.2 kΩcm2 at 37° C and after preincubation in aldosterone; 2) mean transepithelial potential differenceV te was low and variable under standard conditions and at room temperature but increased to −59.5±4.4 mV (sign referring to polarity of apical surface) after preincubation in 10−6 mol/l aldosterone and at 37° C; 3) 10−6 mol/l amiloride added to the apical perfusion fluid largely abolished thisV te while increasingR te by 120%; 4) experiments with 5×10−3 mol/l BaCl2 in the apical perfusion fluid failed to changeR te andV te significantly. This principal cell epithelium therefore has characteristics of a “tight” epithelium with active sodium transport; however, its electrical properties differ from those of the isolated perfused collecting duct segment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00590940
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  • 4
    Electronic Resource
    Electronic Resource
    Interrelationship of renal vascular development and nephrogenesis (1994)
    Springer
    Cell & tissue research 277 (1994), S. 247-257 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Kidney ; Vascular system ; Development ; ontogenetic ; Vasculogenesis ; Endothelium-dectecting antibodies ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Within the cortex region of the neonatal rabbit kidney the developing microvasculature was investigated by means of two endothelium-detecting antibodies (EnPo 1 and EC1). Rows of antibody-labelled cells were found within tissue regions that had previously been described as avascular. We conclude that these vessel-like structures detected by EnPo 1 and EC1 are capillary precursors without lumina. Furthermore, beneath the fibrous capsule within the morphologically homogeneous mesenchyme two cell populations can be discriminated by use of differential antigen expression. The EnPo 1 antigen, which is abundant on endothelial cells and podocytes at different developmental stages, was detected on a subpopulation of mesenchymal cells. These cells were exclusively detected surrounding the tip of the collecting duct ampulla. Due to the unique specificity of EC1 and EnPo 1 the process of microvascular development can be readily followed on serial optical sections gained by laser scan microscopy. (1) Adjacent to EnPo 1-positive mesenchymal cell islets vessel-like structures are found that are in contact with the differentiated vasculature. (2) The renal vesicle is enclosed by a network of vessel-like structures establishing contact with differentiated vessels. (3) No guidance of invading capillary sprouts toward the developing glomerulus and nephron is required, since vascular elements already accompany the earliest detectable nephron stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050152
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  • 5
    Electronic Resource
    Electronic Resource
    Three-dimensional organization of the developing vasculature of the kidney (1996)
    Springer
    Cell & tissue research 287 (1996), S. 193-201 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Kidney ; Development ; Vascular system ; Endothelium-detecting antibodies ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Kidney function depends on a well-developed vascular system. Any impairment of the blood supply disturbs the integrity and function of the organ. The differentiation of renal vessels has been investigation for many years, but little is known about the relationship between nephrogenesis and vessel development. In the present work the spatial organization of the differentiating vessels was analyzed in precisely oriented tissue sections and in optical sections acquired by laser scan microscopy. Developing vessels as well as small capillaries were visualized with two endothelium-detecting antibodies. Small vessels running in parallel towards the organ capsule were detected in numerous cortico-medullary-oriented tissue sections. Cross-sections of the nephrogenic zone showed a regularly arranged network, which was composed of cells detected by both monoclonal antibodies. Parts of this network were localized in regions of the nephrogenic zone which have been assumed to be free of vessels or vessel-like structures for a long time. These results were confirmed by the laser-scan-microscopic analysis of complete cortex explants. The extraordinarily regular arrangement of the endothelial network in the nephrogenic zone allowed us to reconstruct the developing vascular system. The results presented here underline the close relationship between nephrogenesis and vessel development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050745
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  • 6
    Electronic Resource
    Electronic Resource
    Quantitative Bestimmung der Styrol-Metaboliten MandelsÄure und PhenylglyoxylsÄure in Urin mit Hochdruck-Flüssigkeits-Chromatographie und Gas-Chromatographie (1980)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 303 (1980), S. 401-405 
    Details
    ISSN: 1618-2650
    Keywords: Best. von MandelsÄure, PhenylglyoxylsÄure in Harn ; Chromatographie, HPLC/Chromatographie, Gas ; Styrol-Metabolite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es werden eine hochdruckflüssigkeits- und eine gas-chromatographische Methode zur quantitativen Bestimmung der Styrol-Metaboliten MandelsÄure und PhenylglyoxylsÄure im Urin beschrieben. Bei beiden Verfahren wird die PhenylglyoxylsÄure wegen der InstabilitÄt ihrer Derivate durch Oxidation mit Wasserstoffperoxid in der Urinprobe quantitativ zur BenzoesÄure decarboxyliert. Nach einer Flüssig-Flüssig-Extraktion werden die SÄuren mit Diazomethan verestert und dann chromatographiert. Die Probenaufarbeitung erfolgt unter den Bedingungen der internen Standardisierung. Zur Trennung der SÄureester wird bei der GLC ein Temperaturprogramm, bei der HPLC die Gradientenelution mit einer Reversed-Phase-SÄule eingesetzt. Die Detektion wird mit einem Flammenionisationsdetektor bzw. mit einem UV-Detektor mit variabler WellenlÄnge vorgenommen. Mit beiden Methoden wurden Urinproben von 24 styrolbelasteten Personen auf ihren MandelsÄure- und PhenylglyoxylsÄure-Gehalt untersucht. Es ergaben sich Korrelationskoeffizienten von r=0,980 bzw. r=0,916 für Mandelbzw. PhenylglyoxylsÄure.
    Notes: Summary In both the procedures described the urinary phenylglyoxylic acid is quantitatively decarboxylated to benzoic acid by means of an oxidation with hydrogen peroxide. After a liquid-liquid extraction and a subsequent methylation of the acids with diazomethane the chromatographic analysis is carried out. Internal standardization is used for both the methods. The gas chromatographic method uses a temperature program for the separation of the acid esters and a double-flame ionization detector for detection. High-performance liquid chromatography applies gradient elution on a reversed-phase column; a UV-detector with variable wave-length is used for detection. Checking of the reliability of both the methods was done by means of a parallel determination of mandelic and phenylglyoxylic acids in urine samples of 24 persons exposed to styrene. This resulted in a correlation coefficient of r=0.980 and r=0.916 for mandelic and phenylglyoxylic acid, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00470000
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