ISSN:
1432-0983
Keywords:
RNA splicing
;
cox1 gene
;
S. cerevisiae
;
Mosaic genes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have studied the splicing pathway leading to the synthesis of cytochrome oxidase subunit I (COX I) mRNA, by analysing the transcription pattern of several oxi3 − splicing deficient mutants located in the first four introns of the gene. The four introns contain long open reading frames (ORFs) in phase with the upstream exons. All the mutations block the excision of the mutated intervening sequence (IVS) from the pre-mRNA, and accumulate characteristic novel polypeptides of sizes which could correspond to the translation products of the intron's ORE Most of the mutations do not affect the splicing of the following intervening sequences; only in the case of mutations in the all intron is a polar effect observed on the splicing of the second intron, aI2. Our results indicate that the splicing of these two intervening sequences which both belong to the class II of introns described by Michel et al. (1982), is controlled by the activity of the maturases encoded by their respective ORFs and that the translation of the aI2 maturase depends on the previous excision of all IVS. (Moreover, the aI1 maturase, which accumulates in some mutants, can efficiently splice aI2 IVS when the translation of the latter's proper maturase cannot occur).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00389426
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