ISSN:
1434-4726
Keywords:
Human respiratory cilia
;
Ciliary beat
;
Cell culture
;
Differential interference microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The degeneration of ciliary beat of human respiratory cells was studied in monolayer cell cultures by using a differential interference microscope equipped with a high speed video system. This method for studying ciliary beat in cell cultures on collagen-coated cover glasses is quite advantageous, because it allows for detailed study of all parts of ciliary function and not just ciliary beat frequency (CBF). In the present study both CBF and ciliary beat amplitude (CBA) were found to decrease continuously from the 1st day after plating but the wave form of ciliary beat did not change. Cultures with high cell density provided better preservation of normal ciliary beat for a longer period. In contrast, ciliary beat degenerated quickly in cultures with low cell density. CBF and CBA in cell cultures less than 5 days after plating were always high, supporting use of these cultures for studies of normal ciliary motility.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00179306
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