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  • Circular dichroism spectroscopy  (1)
  • Salt-dependence  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of the salt-induced B- to Z-DNA transition (1998)
    Springer
    European biophysics journal 27 (1998), S. 417-423 
    Details
    ISSN: 1432-1017
    Keywords: Key words Z-DNA ; Kinetics ; Circular dichroism ; Salt-dependence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The salt-induced B- to Z-DNA conformational transition is a cooperative- and time-dependent process. From a modified form of the logistic equation which describes an equilibrium between two states we have deduced a kinetic function to quantify the degree of the B to Z transition of a synthetic (dG-dC) ⋅ (dG-dC) polynucleotide. This function was obtained by introduction of time as a variable in the logistic function so that the equilibrium constant, K, is replaced by a new constant K s , characteristic of the type of salt used. This constant is defined as the salt concentration needed to reach the B-Z transition-midpoint in the time unit. The equation fits the data obtained by circular dichroism (CD) for changes in molecular ellipticity of poly(dG-m5dC) ⋅ poly(dG-m5dC) and poly(dG-dC) ⋅ poly(dG-dC) incubated with various concentrations of mono-, di-, and trivalent salts at a constant temperature. The derived expression may be a very useful tool for studying the kinetics of the B- to Z-DNA transition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002490050150
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Empirical validation of a two-state kinetic model for the B-Z transition of double-stranded poly[d(G-m5C)] (1999)
    Springer
    JBIC 4 (1999), S. 759-765 
    Details
    ISSN: 1432-1327
    Keywords: Circular dichroism spectroscopy ; Synthetic polynucleotide ; Salt dependence ; Temperature dependence ; Left-handed DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: 5 C)] in terms of the salt concentration, the absolute temperature, and the cooperativity index. The validity of the formula was tested using circular dichroism spectroscopy after variation of the type of salt (NaCl, MgCl2), the salt concentration, and the temperature of the polynucleotide solution. A consequence of the proposed function is that in conditions of high salt there is a predictable salt threshold which determines the particular molecular mechanism of the B-Z transition. The paper also describes the way in which this threshold level is temperature dependent. A detailed comparison of our data with the experimental data found by other authors is given. The function agrees quantitatively with the experiments and explains the contrasting results found in the literature about the influence in the B-Z transition of both the temperature and the polymer size.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007750050348
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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