ISSN:
1573-6830
Keywords:
Deiters' neuron
;
GABAA receptors
;
Cl− extrusion
;
protein kinase C (PKC) activators
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary 1. Cl− ion outward permeation across microdissected Deiters' neuron plasma membranes is augmented by GABA on the membrane cytoplasmic side. When these neurons are preincubated with a PKC activator, phorbol-12,13-dibutyrate (PdBu), there is a complex pattern of effects on basal and GABA-activated36Cl− in → out permeation. A distinct fact is an increase in basal Cl− passage and a disappearance of the 10−6 M GABA effect at [PdBu] = 0.1µM. 2. Likewise, 0.1µM oleylacetylglycerol (OAG) treatment erases the effect completely, further supporting a role for PKC in modulating GABA-stimulated Cl− in → out permeation. 3. The inactive ester, phorbol-12,13-didecanoate (Pdd), at 0.1µM, does not affect GABA stimulation of Cl− passage. 4. High concentrations (15-20µM) of OAG and PdBu block the “intracellular” GABA effect. However, the 20µM PdBu effect is reversed by 30µM H7. 5. These results indicate a role of endogenous PKC in Cl− extrusion by GABAA receptors on the cytoplasmic side of the Deiters' neuron membrane.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00711463
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