ISSN:
1432-069X
Keywords:
Keratinocytes
;
Interferon-gamma
;
Class II antigens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Explanted human keratinocytes exposed in vitro to recombinant interferon-gamma (IFN-γ) were investigated for the appearance of mRNA for HLA-DR. Using in situ hybridization with a (35S)UTP-labelled HLA-DRβ cRNA probe, mRNA-positive cells were detected already within 6 h with maximal numbers of positive cells as well as the amount of mRNA per cell after 48 h. The corresponding protein HLA-DR, as analysed by immunoperoxidase staining, was detected on 20%–40% of the cells after 24 h and on almost all cells within 48 h. The expression of HLA-DQ and-DP antigens were always exceeded by that of HLA-DR. Whereas an increase in the concentration of IFN-γ above 50 U/ml did not affect the maximal level of HLA-DR reactive cells, there was a fourfold increase in the frequency of cells reactive with HLA-DQ and a twofold increase for HLA-DP when the IFN-γ concentration was raised from 50 to 500 U/ml. When IFN-γ was withdrawn from the cultures, HLA-DR mRNA and protein synthesis ceased — indicating the continuous need for IFN-γ to maintain the HLA-DR synthesis in keratinocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00431060
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