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  • 1
    ISSN: 1432-2048
    Keywords: Clivia ; Cuticle development and fine structure ; Water permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fine structure of the upper cuticular membrane (CM) of Clivia miniata leaves was investigated using electron microscopy. The CM is made up of a thin (130 nm) lamellated cuticle proper (CP) and a thick (up to 7 μm over periclinal walls) cuticular layer (CL) of marbled appearance. Evidence is presented to show that the electron lucent lamellae of the CP do not simply represent layers of soluble cuticular lipids (SCL). Instead, the lamellation is probably due to layers of cutin differing in polarity. It is argued that the SCL in the Cp are the main barrier to water. Thickening of the CM during leaf development takes place by interposition of cutin between the CM and the cellin wall. The cutin of young, expanding leaves has a high affinity for KMnO4 and is therefore relatively polar. As leaves mature, the external CL underneath the CP becomes non-polar, as only little contrast can be obtained with permanganate as the post fixative.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Clivia ; Cuticle (fine structure and development) ; Cutin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of BF3-methanol treatment on the mass and fine structure of isolated Clivia leaf cuticles at different stages of development has been investigated. BF3-methanol cleaves ester linkages in cutin; however, the cuticles are not completely depolymerized. With increasing age, the residue left after BF3-methanol treatment increases in mass. In very young cuticles, 10% of the total cutin resisted BF3-methanol and the fraction of nonester cutin increased up to 62% in mature leaves. Transmission electron microscopy shows that fine structure of the cuticle proper is severely distorted but not destroyed. The internal cuticular layer, which exhibits a heavy contrast when fixed with KMnO4, is completely depolymerized, while the external cuticular layer is hardly affected. The results are discussed in relation to cuticle development and to the function of cuticles as transpiration resistances.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 50 (1967), S. 1267-1274 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Das gas-chromatographische Verhalten der Per-trimethylsilyl-Derivate einiger natürlich vorkommender Uronsäuren bzw. Uronolactone, ihrer Salze, ihrer Reduktions-(Onsäuresalze und-γ-lactone) und Oxydationsprodukte (Glycarsäuren bzw. ihre Salze und Lactone) sowie ihrer Reaktionsprodukte mit Methanol wurde auf den Silikongummi-Kolonnen SE-52 und SE-30 untersucht. Die beim Kochen von D-Mannuron, D-Glucuron und L-Guluronsäure mit 2proz. methanolischer Salzsäure entstehenden Glycosidgemische wurden nach der Silylierung gas-chromatographisch analysiert und die prozentuale Verteilung der auftretenden Pike nach Erreichen des Gleichgewichtes bestimmt. Anhand der gefundenen, auf meso-Erythrit als internen Standard bezogenen Retentionszeiten wird die Trennbarkeit der Uronsäuren diskutiert.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 47 (1964), S. 865-869 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Neben den bisher nach Behandlung von D-Galakturonsäure mit methanolischer Salzsäure in der Wärme gefundenen α- und β-D-galakturonopyranosid-methylestern entstehen grössere Mengen von α- und von β-Methyl-D-galakturono-furanosid-methylester im konstanten Verhältnis von 1 : 3.
    Type of Medium: Electronic Resource
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