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  • Finite Elements  (2)
  • Coat protein  (1)
  • Doubled-haploid lines  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 85 (1993), S. 976-984 
    ISSN: 1432-2242
    Keywords: Kinship coefficient ; Polymerase chain reaction ; Genetic markers ; Hordeum vulgare ; Doubled-haploid lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We investigated random amplified polymorphic DNA (RAPD) in 27 inbred barley lines with varying amounts of common ancestry and in 20 doubled-haploid (DH) lines from a biparental cross. Of 33 arbitrary 10 base primers that were tested, 19 distinguished a total of 31 polymorphisms. All polymorphisms were scored as dominant genetic markers except for 1, where Southern analysis indicated the presence of two codominant amplification products. The inheritance of 19 RAPD polymorphisms and one morphological trait was studied in the DH lines. There was no evidence for segregation distortion, but a group of four tightly linked loci was detected. The frequencies of RAPD polymorphism in pairs of inbred lines were used to compute values of genetic distance (d), which were compared to kinship coefficients (r) between the same pairs of lines. A linear relationship between r and d was evident, but low values of r gave poor predictions of d. Cluster analysis showed that groups of inbred lines based on r were similar to those based on d with some notable exceptions. RAPD markers can be used to gain information about genetic similarities or differences that are not evident from pedigree information.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 89 (1994), S. 583-589 
    ISSN: 1432-2242
    Keywords: Coat protein ; Disease resistance ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Presence of the dominant Tu gene in Lactuca sativa is sufficient to confer resistance to infection by turnip mosaic virus (TuMV). In order to obtain an immunological assay for the presence of TuMV in inoculated plants, the TuMV coat protein (CP) gene was cloned by amplification of a cDNA corresponding to the viral genome using degenerate primers designed from conserved potyvirus CP sequences. The TuMV CP was overexpressed in Escherichia coli, and polyclonal antibodies were produced. To locate Tu on the L. sativa genetic map, F3 families from a cross between cvs “Cobbham Green” (resistant to TuMV) and “Calmar” (susceptible) were genotyped for Tu. Families known to be recombinant in the region containing Tu were infected with TuMV and tested by the indirect enzyme-linked immunosorbent assay (ELISA) using the anti-CP serum. This assay placed Tu between two random amplified polymorphic DNA (RAPD) markers and 3.2 cM from Dm5/8 (which confers resistance to Bremia lactucae). Also, bulked segregant analysis was used to screen for additional RAPD markers tightly linked to the Tu locus. Five new markers linked to Tu were identified in this region, and their location on the genetic map was determined using informative recombinants in the region. Six markers were identified as being linked within 2.5 cM of Tu.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 5 (1985), S. 911-928 
    ISSN: 0271-2091
    Keywords: Incompressible ; Finite Elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We present a numerical procedure to eliminate internal nodes from elements designed to approximate incompressible flow problems. We compare six elements in academic and industrial like flow problem and we discuss their relative qualities. A surprising conclusion is that richer elements may behave less well than simple ones if a good enforcement of incompressibility is not maintained.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 6 (1986), S. 197-218 
    ISSN: 0271-2091
    Keywords: Bingham Fluids ; Forming Process ; Non-Newtonian Flows ; Finite Elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We model the forming process as a fluid flow. A finite element program, FIDAP, which analyses flow problems, was used to calculate velocity and strain rates at points throughout the material during the deformation process. This allows predictions to be made on the shape and quality of the resulting part. The stress-strain relation we used models the plastic flow of metals (Bingham fluids). The FEM approximation of such a fluid is tested by comparing results for a simple analytical example. In forming processes provision must be made for friction between dye and workpiece, and the program was modified accordingly. Two classical ring forming simulations are compared to published results.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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