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  • 1
    Electronic Resource
    Electronic Resource
    Legumin antibodies recognize polypeptides in coated vesicles isolated from developing pea cotyledons (1989)
    Springer
    Protoplasma 150 (1989), S. 79-82 
    Details
    ISSN: 1615-6102
    Keywords: Coated vesicle ; Intracellular transport ; Legumin precursor ; Cotyledons ; Pisum sativum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A highly enriched coated vesicle fraction has been isolated from cotyledons of developing pea seeds. This, and coated vesicles isolated from bovine brain as well as from bean leaves were subjected to SDS-PAGE followed by Western blotting with legumin antibodies. A distinct cross reaction with two polypeptides at around 60 kDa was seen, but only with the coated vesicles isolated from peas. Since legumin is synthesized as a 60 kDa precursor, but occurs as 40 and 20 kDa polypeptides in the protein body, we interpret our results as giving support to the idea that reserve proteins, like lysosomal proteins, are transported via coated vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403662
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The isolation and enrichment of coated vesicles from suspension-cultured carrot cells (1986)
    Springer
    Protoplasma 130 (1986), S. 162-170 
    Details
    ISSN: 1615-6102
    Keywords: Carrot cells ; Coated vesicles ; Isolation ; Ribonuclease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method has been developed to isolate and purify coated vesicles from suspension cultured carrot (Daucus carota L.) cells. It incorporates features of centrifugation methods (sucrose step gradient; Ficoll/D2O gradient) previously employed in the isolation of coated vesicles from mammalian brain tissue. Most important is the treatment of the crude coated vesicle fraction (postmicrosomal supernatant) with ribonuclease to remove ribosomes which are a serious source of contamination in such fractions. The fraction finally obtained is contaminated to the extent of 30% of total observed particles in negatively stained preparations with naked vesicles whose diameter are smaller than those of the coated vesicles. These vesicles are interpreted as being coated vesicles which have been stripped of their coats. SDS-PAGE of coated vesicle fractions purified by this method reveal significant differences in the polypeptide patterns obtained from plant and animal systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276598
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation of a homogeneous coated vesicle fraction from bean leaves (1987)
    Springer
    Protoplasma 136 (1987), S. 154-160 
    Details
    ISSN: 1615-6102
    Keywords: Bean leaves ; Coated vesicles ; Light chains ; Polypeptide patterns
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using a procedure previously developed for suspension-cultured carrot cells, we have been able to isolate two different coated vesicle-containing fractions from green bean leaves (Vicia faba). The two fractions differ in their isopycnic densities in D2O-Ficoll as well as in their diameters. One of the fractions (the less dense of the two) is almost 100% pure as judged by negative staining. Because of this the polypeptide pattern obtained from SDS-PAGE is most clear and has enabled a clear recognition of clathrin light chains, in addition to the 190 kDa heavy chain coat component. Significantly the 100k Da and 50k Da polypeptides typical of brain coated vesicles are absent from bean leaf coated vesicles. Due to a) the high degree of vacuolation b) the presence of large amounts of ribulose bisphosphate carboxylase in the postmicrosomal supernatant, the yield of coated vesicles from bean leaves, as compared to nongreen plant cells, or to bovine brain tissue is extremely low (1 mg coated vesicles from 2.4 kg leaf tissue).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276364
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Two polypeptides (30 and 38kDa) in plant coated vesicles with clathrin light chain properties (1988)
    Springer
    Protoplasma 146 (1988), S. 174-176 
    Details
    ISSN: 1615-6102
    Keywords: Clathrin light chains ; Coated vesicle ; Heat-stability ; Stains-all ; Zucchini
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Coated vesicles have been isolated from bovine brain and etiolated zucchini hypocotyls by centrifugal methods. By putting to use two properties of the light chain polypeptides of brain coated vesicles (calcium binding, heat stability) we have been able to demonstrate the presence of two similar polypeptides with apparent molecular masses of 30 and 38 kDa in plant coated vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01405927
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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