ISSN:
1615-6102
Keywords:
Coated vesicle
;
Intracellular transport
;
Legumin precursor
;
Cotyledons
;
Pisum sativum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A highly enriched coated vesicle fraction has been isolated from cotyledons of developing pea seeds. This, and coated vesicles isolated from bovine brain as well as from bean leaves were subjected to SDS-PAGE followed by Western blotting with legumin antibodies. A distinct cross reaction with two polypeptides at around 60 kDa was seen, but only with the coated vesicles isolated from peas. Since legumin is synthesized as a 60 kDa precursor, but occurs as 40 and 20 kDa polypeptides in the protein body, we interpret our results as giving support to the idea that reserve proteins, like lysosomal proteins, are transported via coated vesicles.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01403662
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