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  • 1
    Electronic Resource
    Electronic Resource
    The complete primary structure of the long form of mouse α1(IX) collagen chain and its expression during limb development
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 1204 (1994), S. 61-67 
    Details
    ISSN: 0167-4838
    Keywords: (Mouse) ; Cartilage ; Chondrocyte ; Collagen ; Endochondral ossification ; Gene expression ; Limb development
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4838(94)90033-7
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  • 2
    Electronic Resource
    Electronic Resource
    Alginate oligosaccharides modulate cell morphology, cell proliferation and collagen expression in human skin fibroblasts in vitro (1999)
    Springer
    Archives of dermatological research 291 (1999), S. 432-436 
    Details
    ISSN: 1432-069X
    Keywords: Key words Alginate oligosaccharides ; Collagen ; Human skin fibroblasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of alginate oligosaccharides on cell proliferation and expression of collagen in cultured skin fibroblasts were studied. The oligosaccharides were found to suppress fibroblast proliferation to half the level in control cultures at a dose of 10 mg/ml during a period of 5 days. The inhibition was accompanied by a change in cell shape. The inhibition of cell proliferation was reversible, since depletion of these oligosaccharides led to a recovery of cell motility. Treatment of confluent cells with 10 mg/ml oligosaccharides for 5 days resulted in a reduction in collagen synthesis to one half of that in control cultures and inhibition of steady state levels of α1(I), α2(I), α1(III) and α1(VI) collagen mRNAs. These results suggest that alginate oligosaccharides are potential modulators of dermal fibroblasts and may provide a useful tool for the treatment of disorders related to abnormal collagen metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050434
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  • 3
    Electronic Resource
    Electronic Resource
    Determination of pheophytinatonickel(II) by reversed-phase highperformance liquid chromatography (1988)
    Springer
    Chromatographia 25 (1988), S. 319-323 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pheophytinatonicke(II) ; Chlorophyll ; Pheophytin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary HPLC determination of pheophytinatonicke(II) (Pheo-Ni) prepared by the replacement of magnesium(II) in chlorophyll with nicke(II) is described. The good separation of PheoNi was obtained by using chemically bonded C18 as the stationary phase and acetone-methanol (50∶50, vol/vol) as the mobile phase. Conventional spectrophotometric method was also used for the determination of PheoNi. For the synthetic samples prepared by mixing (pheophytinato a) nicke(II) [(Pheo-a) Ni] and (pheophytinato b) nicke(II) [(Pheo-b) Ni], analytical values obtained by the spectrophotometric method were very high compared to those obtained by HPLC. In the proposed HPLC method, (Pheo-a) Ni and (Pheo-b). Ni could be determined in the concentration range of 0.028–30μg/ml and 0.038–30μg/ml with relative standard deviations (n=10) of 3.1% and 0.8%, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02324707
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  • 4
    Electronic Resource
    Electronic Resource
    Preparation and determination of manganese(III) chlorophylls by reversed-phase high performance liquid chromatography (1992)
    Springer
    Chromatographia 33 (1992), S. 567-570 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlorophylls ; Manganese(III) chlorophylls
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Manganese chlorophylls have been synthesized by refluxing a mixture of pheophytins dissolved in acetone and manganese(II) acetate anhydride dissolved in glacial acetic acid. A good separation of manganese(III) chlorophylls has been attained by RP-HPLC using chemically bonded C18 silica as a stationary phase and methanol with 3% acetic acid as a mobile phase. An accurate and rapid HPLC method is described for the simultaneous determination of manganese(III) chlorophyll-a [Mn(III)-chl-a] and manganese(III) chlorophyll-b [Mn(III)-chl-b]. Tailing arising from dissociation of acetate ions is improved by addition of sodium acetate (5×10−3 M) to the mobile phase (acetone: methanol=90∶10, vol/vol). The analytical values obtained by the HPLC method are very close to the calculated contents in all samples, but those obtained by spectrophotometry are high because of the interferences from overlapping of absorption bands. In the proposed HPLC method the calibration graphs of Mn(III)-chl-a and Mn(III)-chl-b are linear in the concentration range 0–20 μg cm−3 with relative standard deviations (n=10) of 3.46% and 4.54%, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262249
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  • 5
    Electronic Resource
    Electronic Resource
    Separation and determination of nitrite ion by affinity chromatography with cyano(pheophytinato a)cobalt(III) charged column (1989)
    Springer
    Chromatographia 27 (1989), S. 461-464 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlorophyll-bonded stationary phase ; Cyano(pheophytinato a)cobalt(III) ; Pheophytin a
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The separation and determination of the nitrite ion by affinity chromatography is described. The good separation of the nitrite ion was obtained by using cyano-(pheophytinato a)cobalt(III) bonded octadecylsilica as the stationary phase and water of pH 4.3 adjusted by acetic acid as the mobile phase. Conventional ion chromatograph was also used for the analysis of the nitrite ion. For the synthetic samples prepared by mixing the nitrite and nitrate ions, analytical values obtained by affinity chromatography were in agreement with calculated values and close to the analytical values obtained by ion chromatography. In the proposed affinity chromatography, the nitrite and nitrate ions could be determined in the concentration range of 0.15–1.0ppm and 0.1–1.0ppm with relative standard deviations (n=10) of 5.3% and 4.7%, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02319564
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  • 6
    Electronic Resource
    Electronic Resource
    Preparation and separation of metallobacteriochlorophylla by reversed-phase high-performance liquid chromatography (1996)
    Springer
    Chromatographia 43 (1996), S. 361-364 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bacteriochlorophylla ; Copper(II) bacteriochlorophylla ; Zinc(II) bacteriochlorophylla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Semi-preparative high-performance liquid chromatography has been used for the preparation of copper(II) bacteriochlorophylla [Cu(II)-BChl-a] and zinc(II) bacteriochlorophylla [Zn(II)-BChl-a]. Both compounds are separated on a reversed-phase Inertsil ODS-2 column using a mobile phase of acetone-methanol (25∶75, v/v). The fractionated metallobacteriochlorophylls (M-Bchl-a) are identified by fast atom bombardment mass spectrometry. The spectroscopic parameters such as the wavelength of absorption maxima and the molar extinction coefficients are determined using pure M-Bchl-a obtained by preparative HPLC. The HPLC method proposed here has been demonstrated to be useful for the purification and determination of components of M-Bchl-a.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02271011
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