ISSN:
1432-069X
Keywords:
Progressive systemic sclerosis
;
Lysosomal hydrolases
;
Fibroblast culture
;
Collagen
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary In 14 patients with progressive systemic sclerosis (PSS) the activities of acid lysosomal glycosidases (α-, β-galactosidase, β-glucosidase, β-glucuronidase, and β-N-acetyl-glucosaminidase) were determined fluorometrically in serum, leukocytes, and skin tissue. The β-galactosidase was the only enzyme which exhibited a significantly elevated activity in PSS serum and skin but not leukocytes, as compared to the control. The activity patterns of the studied glycosidases in serum were similar to those found in skin, but differ from the distribution of glycosidase activities in leukocytes. In cultured dermal fibroblasts derived from PSS patients, an elevated intracellular activity of β-galactosidase was detected. These results suggest that the increased β-galactosidase activity in the serum originates from the skin fibroblasts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00431221
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