ISSN:
1615-6102
Keywords:
Cytokinins
;
Daucus carota L.
;
Plant tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Cytokinin-autotrophic strains of carrot callus contained active substances with Chromatographic mobilities on Sephadex LH-20 corresponding to 6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine (Zeatin, Z), 6-(3-methylbut-2-enylamino)purine (iP) and the ribosides (9R)Z and (9R)iP. The apparent major activity was found in a fraction, with an elution volume of 242–291 ml. Hydrolysis of this fraction with HCl and Β-Glucosidase gave rise to Z, indicating that the major active compound is a polar conjugate of Zeatin. In all experiments the extracts were tested immediately after preparation; deep freeze storage, led to a considerable loss of activity in polar fractions. while the free base cytokinins and their ribosides showed increased activity levels. Analogous results were obtained by means of paper chromatography.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01293439
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