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  • 1
    ISSN: 1432-203X
    Keywords: Key words Orchid ; Particle bombardment ; PLB ; Transformation ; Key terminologyProtocorm A small storage organ formed from the germinating embryo ; possessing an apical meristem and a leaf primordium ; protocorm-like body (PLB) A somatic protocorm derived from in vitro culture of apical or axillary bud meristems ; primary PLBs PLBs induced by culturing apical meristem-tips aseptically ; secondary PLBs PLBs formed on the surface of a primary PLB in culture ; proliferate PLBs PLBs proliferating on the surface of either primary or secondary PLBs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A protocol is presented for genetically engineering Cymbidium orchid using particle bombardment. This protocol enabled the routine transformation of orchid plants that were previously difficult to transform. Liquid culture was used to generate a large number of protocorm-like bodies (PLBs) to be bombarded and to promote continued development of the bombarded meristematic tissue. Plasmid DNA (pKH200) carrying the GUS-INT and NPTII genes flanked by tobacco matrix attachment regions was introduced into the meristematic cells of PLBs by particle acceleration. The transformed PLBs were proliferated and selected for kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was then induced from the kanamycin-resistant PLBs, and transgenic plantlets were produced. Both the kanamycin-resistant PLBs and regenerated shoots expressed the GUS-INT gene. The presence of the introduced gene in the transformed orchid plants was confirmed by PCR analysis, sequencing and Southern blot analysis of the PCR product. The recovered transgenic plants were established in soil and acclimatized in the greenhouse.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Key words Poplar ; Populus ; Aspen ; Cottonwood ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of plastic surgery 19 (1996), S. 132-135 
    ISSN: 1435-0130
    Keywords: Microvascular surgery ; Laser Doppler flowmetry ; Computerised monitoring ; Microcirculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Since microvascular procedures are gaining in importance in reconstructive surgery, the need for a specialised monitoring system for detection of vascular compromise, is obvious and storage of data is obligatory. Such a system should be reliable and easy to use. For monitoring purposes, the skin temperature and laser Doppler flowmetry have been elected as the devices which have proven to be the most reliable, with good sensitivity and specitivity. In our computer system these devices can be displayed continuously and simultaneously over a 24-h period. Data of different patients can be stored easily and rapid detection of compromised circulation is achieved. In 1991, 31 microvascular procedures were monitored using this computer system. With this system we have achieved data acquisition, displayed patient's microvascular status and decreased the workload upon nursing and medical staff, this has resulted in an early detection of compromised circulation.
    Type of Medium: Electronic Resource
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