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  • Minitransposon  (2)
  • Confocal laser scanning tomography  (1)
  • Cryotherapy  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 152 (1993), S. 286-290 
    ISSN: 1432-1076
    Keywords: Retinopathy of prematurity, diagnostic procedure ; Cryotherapy ; Scleral buckling ; Vitrectomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Despite advances in ophthalmological care of premature infants, retinopathy of prematurity (ROP) remains a still unsolved problem for paediatricians as well as ophthalmologists. A survey of the current literature concerning drug therapy and surgical management as related to the different stages of ROP is given. The classification system for ROP according to the International Committee is presented as well as our screening policy in relation to the literature. The effectiveness in preventing severe cases of ROP and the toxicity of vitamin E supplementation in high-risk premature infants is still disputed and no recommendations can be given. Cryotherapy is recommended in symmetric cases of stage 3+ ROP. Nevertheless, quite a number of eyes still progress to more severe stages of ROP. Scleral buckling procedures and vitrectomy may lead to anatomical success in a few cases of retinal detachment, however, the visual outcome of such an operation is usually very poor despite reattachment of the cental retina.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 252 (1996), S. 266-274 
    ISSN: 1617-4623
    Keywords: Key words Escherichia coli hemolysin ; Minitransposon ; Secretion of HlyAs-fusion proteins ; TolC protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A simple and efficient procedure for the construction of secreted fusion proteins in Escherichia coli is described that uses a new minitransposon, termed TnhlyAs, carrying the secretion signal (HlyAs) of E. coli hemolysin (HlyA). This transposon permits the generation of random gene fusions encoding proteins that carry the HlyAs at their C-termini. For the construction of model gene fusions we used lacZ, encoding the cytoplasmic β-galactosidase (β-Gal), and phoA, encoding the periplasmic alkaline phosphatase, as target genes. Our data suggest that all β-Gal-HlyAs fusion proteins generated are secreted, albeit with varying efficiencies, by the HlyB/HlyD/TolC hemolysin secretion machinery under Sec-proficient conditions. In contrast, the PhoA-HlyAs fusion proteins are efficiently secreted in a secA mutant strain only under SecA-deficient conditions.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 252 (1996), S. 266-274 
    ISSN: 1617-4623
    Keywords: Escherichia coli hemolysin ; Minitransposon ; Secretion of HlyAs-fusion proteins ; TolC protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple and efficient procedure for the construction of secreted fusion proteins inEscherichia coli is described that uses a new minitransposon, termed TnhlyAs, carrying the secretion signal (HlyAs) ofE. coli hemolysin (HlyA). This transposon permits the generation of random gene fusions encoding proteins that carry the HlyAs at their C-termini. For the construction of model gene fusions we usedlacZ, encoding the cytoplasmicβ-galactosidase (β-Gal), andphoA, encoding the periplasmic alkaline phosphatase, as target genes. Our data suggest that allβ-Gal-HlyAs fusion proteins generated are secreted, albeit with varying efficiencies, by the HlyB/HlyD/TolC hemolysin secretion machinery under Sec-proficient conditions. In contrast, the PhoA-HlyAs fusion proteins are efficiently secreted in asecA mutant strain only under SecA-deficient conditions.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Der Ophthalmologe 94 (1997), S. 673-677 
    ISSN: 1433-0423
    Keywords: Schlüsselwörter Papillenschwellung ; Heidelberg-Retina-Tomograph ; Pseudotumor cerebri ; Verlaufskontrolle ; Key words Papilledema ; Optic disk swelling ; Confocal laser scanning tomography ; Follow-up
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: The Heidelberg retina tomograph (HRT) is a new instrument to analyze the three-dimensional structure of the retina. It is based on confocal laser scanning technology. Scientific interest has so far focused on the follow-up of glaucomatous optic nerve head damage. As parameters such as cup depth and cup volume can be reproduced with a high degree of accuracy, this new instrument may prove to be an excellent tool to follow up swelling of the optic disk. Patients and Method: A total of 21 patients suffering from optic disk swelling of various etiologies were included in a pilot study. Measurements were taken at six different time points. The maximum optic disk elevation and the volume of swelling were evaluated. Visual acuity and the visual field were determined, and fundus photographs were taken. Results: A reduction in optic disk swelling over time may be demonstrated by both morphological parameters and correlates with improvement in fundus changes. The change in the volume of swelling is greater than the change in maximum disk elevation. The course of optic disk swelling differs between patients with anterior ischemic optic neuropathy and those with pseudotumor cerebri. No correlation was found between our measurements with the HRT and functional parameters (visual acuity and visual field). Conclusion: The HRT is a good tool for the follow-up of optic disk swelling, particularly optic disk elevation due to increased intracranial pressure.
    Notes: Der Heidelberg-Retina-Tomograph (HRT) ist ein neues Instrument zur Darstellung der dreidimensionalen Topographie des Augenhintergrunds, basierend auf dem Prinzip der konfokalen Laser-Scanning-Tomographie. Aufgrund der guten Reproduzierbarkeit topographischer Meßparameter im Rahmen der Glaukomdiagnostik erscheint er auch als zuverlässiges Verfahren zur Verlaufsdokumentation einer Papillenprominenz geeignet. Patienten und Methode: Es wurden mit dem Heidelberg-Retina-Tomographen 21 Patienten mit einer Papillenschwellung unterschiedlicher Genese im Verlauf dokumentiert. Die Papillenmorphologie wurde zu 6 Untersuchungszeitpunkten aufgezeichnet. Ausgewertet wurden die maximale Papillenprominenz sowie ihr Volumen. Als weitere Kontrollparameter dienten Visus, Perimetrie sowie Fundusfotografien. Ergebnisse: Die Abnahme der Papillenschwellung läßt sich anhand beider topographischer Meßparameter darstellen und korreliert mit dem klinischen Fundusbefund. Das Volumen der geschwollenen Papille zeigt im Vergleich zur maximalen Höhe der Papille im Mittel stärkere Veränderungen. Im frühen Krankheitsverlauf nimmt das Schwellungsvolumen bei Patienten mit anteriorer ischämischer Optikopathie zu, während es beim Pseudotumor cerebri rückläufig ist. Eine sichere Korrelation mit funktionellen Parametern (Visus, Gesichtsfeld) ergibt sich nicht. Schlußfolgerung: Der Heidelberg-Retina-Tomograph eignet sich zur objektiven Verlaufskontrolle von Papillenschwellungen unterschiedlicher Genese, insbesondere jedoch zur Dokumentation und Therapiekontrolle von Stauungspapillen im Rahmen eines Pseudotumor cerebri.
    Type of Medium: Electronic Resource
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