ISSN:
1432-2307
Keywords:
Cytokeratin
;
Intermediate Filament Proteins
;
Conformation-dependent epitopes
;
Immunocytochemistry
;
Tumor diagnosis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The epitope recognized by the murine monoclonal antibody (mAB lu-5) recently described as a formaldehyde-resistant, “pan-epithelial marker” of great value in tumour diagnosis is located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution from amphibia to man. As this epitope is not reactive after SDS-polyacrylamide gel electrophoresis (SDS-PAGE), the epitope-bearing protein has been identified by a dot-blot antibody binding assay, using purified proteins in which the epitope is reconstituted. We show that the epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins. The location of this widespread epitope is discussed with respect to homologies of amino acid sequences of cytokeratins and their conformations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00712737
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