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  • 1
    Electronic Resource
    Electronic Resource
    G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae (1990)
    Springer
    Current genetics 18 (1990), S. 303-313 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; G418 resistance ; Gene cartridges ; Heterologous Gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Coding sequence cartridges for aminoglycoside phosphotransferase (APT) were isolated from bacterial transposon Tn903. When incorporated into a heterologous gene construction utilising the PGK1 promoter and terminator, the heterologous APT gene provided a G418-resistance determinant that functioned efficiently as a dominant marker for yeast in both multiple- and single-copy. Transformant colonies on selective medium appeared rapidly, within 36–48 h, and growth rate of the transformed cells was normal. A simple and highly sensitive radiolabelling assay for APT enzyme activity was developed for use with crude cell protein extracts. Enzyme activity units were equated to the amount of APT protein present in the cells, and the APT protein was shown to be stable in yeast. Heterologous APT expression was 130-fold reduced compared with homologous PGK1. This resulted from an estimated two-fold decrease in mRNA level and a 65-fold decrease in translation efficiency. The latter was unaffected by AUG sequence context change, but corresponded with a high frequency of minor codons in the APT-coding sequence. APT can be used as a semi-quantitative reporter of gene expression, whose useful features are in vivo detection via the G418-resistance phenotype and powerful cell-free assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318211
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The induction of dynamical recognizers (1991)
    Springer
    Machine learning 7 (1991), S. 227-252 
    Details
    ISSN: 0885-6125
    Keywords: Connectionism ; language ; induction ; dynamics ; fractals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A higher order recurrent neural network architecture learns to recognize and generate languages after being “trained” on categorized exemplars. Studying these networks from the perspective of dynamical systems yields two interesting discoveries: First, a longitudinal examination of the learning process illustrates a new form of mechanical inference: Induction by phase transition. A small weight adjustment canses a “bifurcation” in the limit behavior of the network. This phase transition corresponds to the onset of the network's capacity for generalizing to arbitrary-length strings. Second, a study of the automata resulting from the acquisit on of previously published training sets indicates that while the architecture is not guaranteed to find a minimal finite automaton consistent with the given exemplars, which is an NP-Hard problem, the architecture does appear capable of generating non regular languages by exploiting fractal and chaotic dynamics. I end the paper with a hypothesis relating linguistic generative capacity to the behavioral regimes of non-linear dynamical systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00114845
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The Induction of Dynamical Recognizers (1991)
    Springer
    Machine learning 7 (1991), S. 227-252 
    Details
    ISSN: 0885-6125
    Keywords: Connectionism ; language ; induction ; dynamics ; fractals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A higher order recurrent neural network architecture learns to recognize and generate languages after being “trained” on categorized exemplars. Studying these networks from the perspective of dynamical systems yields two interesting discoveries: First, a longitudinal examination of the learning process illustrates a new form of mechanical inference: Induction by phase transition. A small weight adjustment causes a “bifurcation” in the limit behavior of the network. This phase transition corresponds to the onset of the network's capacity for generalizing to arbitrary-length strings. Second, a study of the automata resulting from the acquisition of previously published training sets indicates that while the architecture is not guaranteed to find a minimal finite automaton consistent with the given exemplars, which is an NP-Hard problem, the architecture does appear capable of generating non-regular languages by exploiting fractal and chaotic dynamics. I end the paper with a hypothesis relating linguistic generative capacity to the behavioral regimes of non-linear dynamical systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022651113306
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Differential gene expression in CD3e- and RAG1-deficient thymuses: definition of a set of genes potentially involved in thymocyte maturation (1999)
    Springer
    Immunogenetics 50 (1999), S. 255-270 
    Details
    ISSN: 1432-1211
    Keywords: Key words Thymus ; T lymphocytes ; Gene regulation ; Chemokines ; Thymic stroma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A set of 3000 mouse thymus cDNAs was analyzed by extensive measurement of expression using complex-probe hybridization of DNA arrays ("quantitative differential screening"). The complex probes were initially prepared using total thymus RNA isolated from C57BL/6 wild-type (WT), CD3e- and RAG1-deficient mice. Over 100 clones displaying over- or under-expression by at least a factor of two between WT and knockout (KO) thymuses were further analyzed by measuring hybridization signatures with probes from a wide range of KO thymuses, cell types, organs, and embryonic thymuses. A restricted set of clones was selected by virtue of their expression spectra (modulation in KO thymuses and thymocytes, lymphoid cell specificity, and differential expression during embryonic thymus development), sequenced at one extremity, and compared to sequences in databases. Clones corresponding to previously identified genes (e.g., Tcrβ, Tcf1 or CD25) showed expression patterns that were consistent with existing data. Ten distinct clones corresponding to new genes were subjected to further study: Northern blot hybridization, in situ hybridization on thymus sections, and partial or complete mRNA sequence determination. Among these genes, we report a new serine peptidase highly expressed in cortical epithelial cells that we have named thymus-specific serine peptidase (TSSP), and an acidic protein expressed in thymocytes and of unknown function that we have named thymus-expressed acidic protein (TEAP). This approach identifies new molecules likely to be involved in thymocyte differentiation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050601
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    Paper (German National Licenses)
    Fulltext
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