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  • Corynebacterium  (1)
  • Keywords: Corynebacterium glutamicum; glutamate; intracellular concentrations; carbon fluxes  (1)
  • Succinoglucan  (1)
  • cofactors balances  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Intra- and extracellular concentrations of glutamate, lactate and acetate during growth of Corynebacterium glutamicum on different media (1997)
    Springer
    Journal of industrial microbiology and biotechnology 18 (1997), S. 312-318 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: Corynebacterium glutamicum; glutamate; intracellular concentrations; carbon fluxes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Corynebacterium glutamicum ATCC 17965 was cultivated in a 4-L batch aerated fermentor with glucose, fructose and mixtures of these two sugars in various proportions as carbon sources and with different concentrations of minerals and vitamins. A multilayer centrifugation technique was devised to obtain cell extracts in order to assess intracellular production of glutamate and partitioning between intracellular and extracellular spaces for lactate and acetate, the main by-products produced during the growth phase. Glutamate production increased with the proportion of glucose in the carbon source. The average value for the intracellular concentration of glutamate obtained with basic glucose medium was increased three-fold when initial concentrations of vitamins and minerals were increased four-fold. In this case, overall production of glutamate (16.3 mM) reached the highest value obtained. Production of acetate was weak on all media types (〈 1.6 mm). it was the same for lactate synthesis in media where glucose remained the major carbon source (〈 2.3 mm). production of lactate was significantly higher on media where fructose was the main carbon source (〉 10 mM to 60 mM). The increase in lactate production and the decrease in glutamate production were correlated to a modification of carbon flux distribution between the metabolic pathways as the fructose proportion was increased. An increase in the concentration of minerals favoured production of glutamate during growth. This was correlated with an increase in the NADPH,H+ production rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900386
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Modeling growth and succinoglucan production by Agrobacterium radiobacter NCIB 9042 in batch cultures (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 65-74 
    Details
    ISSN: 0006-3592
    Keywords: Succinoglucan ; Agrobacterium radiobacter ; Structured model ; ATP ; cofactors balances ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Wild-type Agrobacterium radiobacter NCIB 9042 has been cultivated in batch cultures on a synthetic medium which was adapted for growth and succinoglucan production. Experiments were carried out in a 4-L stirred-tank aerated reactor. Glucose, biomass, polysaccharide, protein, and inorganic- and organic-nitrogen concentrations were measured, and oxygen consumption and CO2 production rates were obtained by a gas-balance technique. Nitrogen balance shows that inorganic nitrogen is entirely recovered into proteins. The carbon balance is satisfied with in ±5%. Stoichiometric equations for biomass growth and succinoglucan synthesis were established. The biosyntheticpolymer pathways including ATP and cofactor consumption were investigated. From previous studies, a (P/O) value of 1.66 is selected for oxygen sufficient cultures. The actual ATP requirements of 25.4 mmol ATP/g succinoglucan (38.5 mol ATP/mol succinoglucan), determined by a metabolic analysis, is 2.39 times the stoichiometric value. Experimental results were modeled by a system of differential equations. The exponential growth phase was described by a nitrogen-limited Monod equation. Subsequent succinoglucan synthesis followed a slightly modified Luedeking-Piret relation partitioning internal and external polysaccharide. Experimentally determined coefficients are compared with published results for continuous culture of A. radiobacter NCIB 11883.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260380109
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Metabolic flux distribution in Corynebacterium melassecola ATCC 17965 for various carbon sources (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 51 (1996), S. 177-189 
    Details
    ISSN: 0006-3592
    Keywords: Corynebacterium ; intracellular flux ; metabolism network ; growth phase ; mixture of carbon sources ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The distribution of carbon in the metabolic network of a bacterial cell was estimated by a mass-balance-based intracellular flux computation method. It was applied to the growth phase of Corynebacterium melassecola, a glutamic acid producing bacterium, using experimental production yields of biomass, lactate and acetate measured during batch cultures on glucose, fructose, and various mixtures of both sugars. This flux computation method identifies the direction of the 86 reactions that ensure proper metabolic function during the growth phase of C. melassecola. Flux ratios allow comparison of calculated and relevant experimental yields. The results highlight the key influence of the biomass production yield YX-O2 on the overall distribution of carbon; the proportion of carbon drained in the pentose-P pathway fell from a value in the range of 54% to 47% on media containing glucose (YX-O2 = 1.75 to 1.56 g X/g O2) to 37% on fructose medium (YX-O2 = 1.36 g X/g O2). The highest maintenance requirement was calculated on fructose medium (Jm = 290 mol ATP/100 mol fructose) which must be connected to a lower efficiency of cell multiplication observed on this substrate. Another important result was that the significant decreases in experimental values of production yields and rates observed on fructose medium which were related to the operation of the FBPase. In particular, it was estimated that, as long as the proportion of glucose in the carbon source remains above 22% (78% fructose), the operation of the FBPase is not necessary and the bacteria exhibit behavior similar to that observed on glucose alone; this result is consistent with experimental observations. © 1996 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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