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  • Coupled columns  (2)
  • Electrochemical detection  (1)
  • Enantiomers  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Coupled columns in bioanalytical work
    Amsterdam : Elsevier
    Journal of Pharmaceutical and Biomedical Analysis 4 (1986), S. 181-189 
    Details
    ISSN: 0731-7085
    Keywords: Coupled columns ; bioanalysis. ; liquid chromatography
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0731-7085(86)80040-1
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Automated analysis of terbutaline (BricanylR) in human plasma with liquid chromatography and electrochemical detection using column-switching (multidimensional chromatography) (1982)
    Springer
    Chromatographia 16 (1982), S. 341-344 
    Details
    ISSN: 1612-1112
    Keywords: Terbutaline ; Plasma ; Automated HPLC ; Column-switching ; Electrochemical detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An automated method based on liquid chromatography has been developed for the determination of terbutaline in human plasma in the range of 5–50 pmole·ml−1. The necessary sensitivity and selectivity was obtained by using electrochemical detection and a microprocessor-controlled column switching system. A combination of three columns was used: a C8 type for pre-separation, a C18 type for trapping and, for final separation, a strongly acidic ion exchanger. The accuracy of the method was examined by comparison with a method based on gas chromatography — mass spectrometry. The overall precision was ±3.5% and ±2.2% respectively at 5 and 50 pmole·ml−1. The total absolute recovery for terbutaline and internal standard at the above concentration levels were in the range 85–106%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258934
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Chiral separation on achiral stationary phases with different functionalities using β-cyclodextrin in the mobile phase and application to bioanalysis and coupled columns (1991)
    Springer
    Chromatographia 32 (1991), S. 215-223 
    Details
    ISSN: 1612-1112
    Keywords: Liquid chromatography ; Coupled columns ; Cyclodextrin ; Enantiomers ; Chiral separation ; Bioanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary β-cyclodextrin was used in the mobile phase as chiral selector for separating the enantiomers of terbutaline, chlorthalidone and oxazepam. The effect on chiral resolution using e.g. hydrophobic, polar or cation exchanging stationary phases was investigated. Both the chiral separation factor and retention level were affected by the concentration of methanol and β-cyclodextrin. The stationary phase had no effect on the chiral separation only on the level of retention. By tuning the concentration of β-cyclodextrin and methanol in the mobile phase chiral separation could be obtained on most stationary phases. By changing the stationary phase while adjusting the mobile phase composition to maintain the chiral selectivity, improvements of the selectivity towards e.g. endogenous compounds can be obtained when separating enantiomers in complex matrixes as biological fluids. Further improvement on selectivity can be obtained if coupled columns are used. This is examplified for separation of chlorthalidone and terbutaline enantiomers in biological fluids by coupling an achiral column to another achiral column and using a mobile phase containing β-cyclodextrin on the last column.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02276243
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    Paper (German National Licenses)
    Fulltext
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