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  • 1
    Digitale Medien
    Digitale Medien
    Stability and expression of transferred DNA in F1 tobacco transformants studied at various states of differentiation (1987)
    Springer
    Planta 172 (1987), S. 448-462 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Agrobacterium ; Crown gall ; Nicotiana (crown gall, T-DNA) ; Transformation (tobacco) ; Transferred DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Grafts from the SR1 tobacco crown-gall lines NT1 (having a deletion eliminating part of the transferred (TL)-DNA auxin locus) and NT2 (having an IS60 insertion in gene 2 of this auxin locus) were cross-pollinated with pollen from nontransformed SR1 tobacco plants. One half of the resulting F1 progeny resembled the female parent (“transformed” NT1-like and NT2-like seedlings respectively) and one half resembled the male parent (“non-transformed” SR1-like seedlings). For three states of differentiation (callus, shoot, graft) all phenotypic markers of the transformed seedlings studied were identical to those of the transformed female parent. Most phenotypic markers of non-transformed seedlings corresponded with markers of the male parent. Unlike the SR1 male parent, however, the SR1-like seedlings showed the maternal traits hyperstyly and male sterility. These two traits were inherited by 100% of the F1 seedlings studied. Ninety percent of the non-transformed F2 seedlings were still male-sterile whereas in as much as 50–100% of the non-transformed F3 progeny, male fertility had been restored. The SR1-like F1 seedlings did not contain any T-DNA. At the level of restriction-fragment analysis the T-DNA structures of all 22 NT1-like seedlings examined were identical to the T-DNA structure of their female parent NT1. The steady-state level of transcripts 4 (cytokinin locus) and 6a/6b relative to transcript 3 (octopine-synthase locus) was less in shoots and grafts than in callus. Observed variation in shoot morphology among the twenty-two NT1-like seedlings was not correlated with T-DNA structure, organization and expression at the level of steady-state mRNA. The T-DNA structure of NT2 and its transformed seedlings deviated from regular border-to-border TL-DNA, in that it extended beyond the left border repeat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00393860
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  • 2
    Digitale Medien
    Digitale Medien
    The expression of tumour markers in intraspecific somatic hybrids of normal and crown gall cells from Nicotiana tabacum (1980)
    Springer
    Theoretical and applied genetics 56 (1980), S. 203-208 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Somatic hybrids ; Tumour markers ; Crown gall ; Protoplast fusion ; T-DNA ; Tobacco
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Following fusion of protoplasts from crown gall tumour calli, characterized by hormone independent growth, and protoplasts from normal tissues of a streptomycin-resistant mutant, SR1, we selected hormone independent streptomycin-resistant calli in Nicotiana tabacum. The tumour line, B6S3, lost the ability to form shoots. Some of the selected lines, similar to SR1, however, are morphogenic. Both calli and shoots contained the tumour specific enzyme lysopinedehydrogenase. The hybrid shoots are resistant to Agrobacterium infection and do not root. These tumorous properties are dominantly expressed in the somatic hybrids.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00295450
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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