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  • 1
    Electronic Resource
    Electronic Resource
    In vitro protein synthesis and α amylase activity in F cells from hepatopancreas ofPalaemon serratus (Crustacea; Decapoda) (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 272-277 
    Details
    ISSN: 1420-9071
    Keywords: Crustacean ; prawn ; hepatopancreas ; cell suspension ; protein synthesis ; α-amylase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawnPalaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with3H-leucine, incorporation was highest in the F cell fractions. Measurements of α-amylase activity, indicated that the two populations of F cells may be derived from the same cell type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01930474
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dissociated cell suspensions ofCarcinus maenas Y-organs as a tool to study ecdysteroid production and its regulation (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 153-158 
    Details
    ISSN: 1420-9071
    Keywords: Ecdysteroid biosynthesis ; neuropeptide regulation ; Y-organs ; crustacea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In vitro ecdysteroid production by dissociated Y-organ cells of the shore crabCarcinus maenas was characterized during short-term incubations. Under optimized conditions (M199 adjusted to crab osmolality and with the addition of 10% foetal calf serum), ecdysteroid production by dispersed cells increased linearly during 4-hour incubations, with little intra-assay variation. 25-deoxyecdysone was mainly produced. PurifiedCarcinus molt inhbiting hormone (CamMIH) produced a dose-dependent inhibition of ecdysteroid production by dispersed cells. The cells were about 50 times more sensitive than whole glands to MIH. Other structurally-related peptides were tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01984955
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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