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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of epidemiology 10 (1994), S. 215-218 
    ISSN: 1573-7284
    Keywords: Cryptosporidium oocysts ; Staining methods ; Water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A study was carried out on the presence of oocysts ofCryptosporidium spp. in water for human consumption in the Province of Salamanca, Spain, using four different staining techniques: Ziehl-Neelsen, auramine, indirect immunofluorescence with monoclonal antibodies (Meridian, USA) and direct immunofluorescence with monoclonal antibodies (Pasteur, France). The presence of oocysts was detected in the water used for the washing of sand filters in the four water purifying plants and in the drinking water of two of the six geographical areas studied. The Ziehl-Neelsen and auramine staining techniques were found to have excellent diagnostic correlation. Immunofluorescence stainings with monoclonal antibodies proved to be less sensitive.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-7284
    Keywords: Seasonal prevalences ; Cryptosporidium spp. ; Giardia intestinalis ; Day care centres
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Five studies were carried out in children younger than 4 years old attending regularly day care centres in order to determine the intestinal parasitization by Cryptosporidium and Giardia. The percent of children parasitized by Cryptosporidium was 10% (17 children out of 170 studied). Cryptosporidiosis was more frequent in winter than in other seasons (difference statistically significant). Giardia intestinulis which was the most frequent parasite, was identified in 25.3% (43 children out of 170 studied). Giardiasis was more frequent in autumn and in the low SEL (difference statistically significant in both cases). The frequency of giardiasis increased from 8% in children studied during 1–2 seasons, to 34% in children studied for 3–5 seasons (difference statistically significant), whereas the frequency of cryptosporidiosis did not show variation with increased number of specimens studied per child. The 35% of children parasitized by Cryptosporidium sp. showed gastrointestinal symptoms but only 14% of children with Giardia intestinalis.
    Type of Medium: Electronic Resource
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