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  • Cyanide pathway  (1)
  • Root (asparagine synthesis)  (1)
  • maize  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Enzymes of asparagine synthesis in maize roots (1979)
    Springer
    Planta 146 (1979), S. 237-241 
    Details
    ISSN: 1432-2048
    Keywords: Asparagine ; Cyanide pathway ; Root (asparagine synthesis) ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An asparagine synthetase which is active with either glutamine or NH 4 + has been found in maize (Zea mays L.) roots. Unlike the enzyme obtained from legume cotyledons, the maize-root enzyme is only slightly more efficient with glutamine (Km, 1.0 mM) than with NH 4 + (Km, 2.0–3.0 mM). The activity of this enzyme is higher in the mature root than in the root-tip region, i.e. root cells develop a capacity to make asparagine from glutamine or NH 4 + as they mature. β-Cyanoalanine synthetase is also present in maize roots. The apparent Km for cysteine is 2.6 mM and for cyanide is 0.57 mM. The enzyme is more active in the root tip than in mature root tissue. Thus, if asparagine were made in the root tip, the cyanide pathway could represent the mechanism of synthesis. It is our contention, however, that this potential is not realized under normal conditions because 14C-experiments performed previously have indicated a limited availability of both CN and cysteine in the maize root.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388238
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid methods for quantifying VAM fungal infections in maize roots (1992)
    Springer
    Plant and soil 147 (1992), S. 317-319 
    Details
    ISSN: 1573-5036
    Keywords: bioassay ; maize ; root pigmentation ; vesicular arbuscular mycorrhiza
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Roots of maize (Zea mays cv W64A × W182E) infected by vesicular arbuscular mycorrhizal (VAM) fungi (Glomus versiforme (Karst) Berch or a Glomus species isolated from an alfalfa soil) exhibit a bright yellow pigmentation. The percentage of pigmented roots can be quantified by a rapid visual estimate or by a grid intersect method. Both methods gave similar estimates of VAM infection to those obtained using a grid intersect count on cleared roots stained with chlorazol black E. Thus for experimental or field evaluation where speed and quantity are important, the rapid visual estimate (less than one minute for each washed root system) yields reliable results. The yellow root intersect method takes longer (5–15 minutes per root system) but gives more reproducible results. The yellow root pigmentation is light sensitive However, root systems can be reliably assayed after 1 week when stored at 5°C in the dark or after 1 year if dried.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029083
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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