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  • Neurospora  (2)
  • Cyanide-sensitive and insensitive respirations  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Genetic analysis of transformation in a microconidiating strain of Neurospora crassa (1985)
    Springer
    Current genetics 10 (1985), S. 313-320 
    Details
    ISSN: 1432-0983
    Keywords: Transformation ; Neurospora ; Microconidia ; Plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have characterized Neurospora crassa transformants obtained with plasmid pDV1001 bearing the cloned catabolic dehydroquinase (qa-2 +) gene (Hughes et al. 1983) and fluffy 268 host strain producing only uninucleate microconidia allowing to isolate individual transformation products. The percentage of transformed nuclei in the mycelium and their stability were determined by genetic analysis of microconidia produced on selective or non-selective medium. About half of the transformants originating from mycelial spheroplasts were apparently homokaryotic. Catabolic dehydroquinase activity was in agreement with the proportion of transformed nuclei. The DNAs from four transformants analyzed by Southern hybridization showed restriction fragments expected for integration of pDV1001 into genomic DNA by non-homologous recombination. No plasmids could be rescued from the undigested DNAs of the transformants by transformation of E. coli. One transformant, 8268-6, was unstable and generated a high proportion of segregants. Plasmid pDV1001 sequences were absent in their DNA. Colonies originating from microconidia of strain fl268-6 on selective plates often lost the transformed character. These results suggest that instability in this transformant is due to the loss of integrated plasmid sequences during vegetative growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365627
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Heat-induced changes in respiratory pathways and mitochondrial structure during microcycle conidiation of Neurospora crassa (1980)
    Springer
    Archives of microbiology 125 (1980), S. 53-58 
    Details
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; Microcycle conidiation ; Heat-treatment ; Cyanide-sensitive and insensitive respirations ; Mitochondrial structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in both respiratory pathways and mitochondrial structure of Neurospora crassa occurred under conditions of microcycle conidiation. Upon heat-treatment at 46°C, conidia developed a highly cyanide-insensitive, hydroxamate-sensitive respiration associated with morphological alterations in mitochondrial membranes; such changes were time-dependent. When heat-treated conidia were shifted down to 25°C, the alternate, hydroxamate-sensitive respiration decreased significantly, paralleling the recovery of well-cristated mitochondria with an electron-dense matrix in the germ tubes. The decrease in hydroxamate-sensitivity was associated with two periods of increase in cyanide sensitivity corresponding to the events of germination and precocious proconidial budding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403197
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural demonstration of loss and recovery of cytochrome oxidase activity during and after heat induction of microcycle conidiation inNeurospora crassa (1983)
    Springer
    Protoplasma 116 (1983), S. 149-154 
    Details
    ISSN: 1615-6102
    Keywords: Cytochrome oxidase ; Cytochemistry ; Ultrastructure ; Neurospora ; Heat treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytochrome oxidase (EC 1.9.3.1) activity of conidia of wild-typeNeurospora crassa was detected cytochemically at the ultrastructural level by 3,′-diaminobenzidine (DAB) reaction during heat treatment at 46 °C and after shift down to 25 °C. At 46 °C the decrease in the enzyme activity was shown to be time-dependent (0–10 hours) in the progressively overswelling conidia. Gradual recovery of the DAB reaction occurred in mitochondria of conidia outgrowing conidiogenous germ tubes (microcycle conidiation) when shifted down to 25 °C. Inactivation of cytochrome oxidase was efficiently prevented during heat treatment in the presence of Tween 20. Such conidia no longer overswelled but still required shift down to 25 °C to produce germ tubes with delayed conidiogenous ability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279832
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    Paper (German National Licenses)
    Fulltext
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