ISSN:
1432-1912
Keywords:
Human platelets
;
Beta-adrenoceptors
;
Cyclic AMP accumulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The presence of beta2 adrenoceptors on the human platelet is well established although the coupling of this receptor to adenylate cyclase is disputed. In this study of highly purified platelets isoprenaline did not stimulate whole platelet cyclic AMP alone or in the presence of forskolin. Minimal contamination of the purified platelets (approximately 0.05%) with mononuclear white blood cells may lead to the spurious appearance of isoprenaline stimulated cAMP. These results confirm our previous findings of lack of coupling of the human platelet beta2 adrenoceptor to adenylate cyclase and indicate the importance of purity of preparation for platelet biochemical studies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00169254
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