ISSN:
1432-1327
Keywords:
Key words Azurin
;
Copper-A
;
Cyclic voltammetry
;
Protein folding
;
Rack-induced coordination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Cyclic voltammetry has been used to determine the reduction potentials of blue (Pseudomonas aeruginosa azurin) and purple (Thermus thermophilus CuA domain) copper proteins unfolded by guanidine hydrochloride. These Cu(II/I) potentials [456 (azurin); 453 (CuA) mV vs., NHE] are higher than those of the folded proteins. The downshift of the potential in the folded state can be accounted for by assuming that rack-induced axial coordination stabilizes Cu(II) relative to Cu(I) in a protein-encapsulated active site.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007750050246
Permalink